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生物质化学工程 ›› 2010, Vol. 44 ›› Issue (2): 9-14.

• 论文 • 上一篇    下一篇

四株酵母乙醇发酵的初步研究

朱均均1, 勇强1, 陈尚钘1,2, 张春娟1, 徐勇1, 余世袁1   

  1. 1. 南京林业大学, 林木遗传与生物技术省部共建教育部重点实验室, 江苏 南京 210037;2. 江西农业大学, 林学院, 江西 南昌 330045
  • 收稿日期:2009-09-27 修回日期:1900-01-01 出版日期:2010-03-30 发布日期:2010-03-30
  • 通讯作者: 余世袁,博士,教授,博士生导师,主要从事林产资源生物化学加工的研究;E-mail:syu@njfu.edu.cn。

Preliminary Study on Ethanol Fermentation by Four Strains

ZHU Jun-jun1, YONG Qiang1, CHEN Shang-xing1,2, ZHANG Chun-juan1, XU Yong1, YU Shi-yuan1   

  1. 1. Key Laboratory of Forest Genetics & Biotechnology, Ministry of Education, Nanjing Forestry University, Nanjing 210037, China;2. College of Forestry, Jiangxi Agricultural University, Nanchang 330045, China
  • Received:2009-09-27 Revised:1900-01-01 Online:2010-03-30 Published:2010-03-30

摘要: 比较了休哈塔假丝酵母NLP21、树干毕赤酵母NLP22、NLP23和NLP31,在30 g/L的木糖和混合糖(葡萄糖15 g/L+木糖15 g/L)发酵培养基上以及在培养基中氮源浓度降低到原来1/2和1/10时的发酵性能。结果表明,在30 g/L木糖发酵培养基上,NLP23和NLP31产乙醇质量浓度最高,分别为(11.14±0.13)和(11.15±0.08) g/L。在15 g/L葡萄糖+15 g/L木糖混合糖发酵培养基上,NLP31产乙醇质量浓度最高,为(10.91±0.12) g/L。当发酵培养基中氮源浓度降低到原来的1/2时,NLP23和NLP31产乙醇能力相当,但后者产木糖醇的量增大;当氮源质量浓度降低到原来的1/10时,NLP23和NLP31产乙醇能力随着发酵轮数的增加,逐渐下降,氮源浓度低,降低了乙醇的产量。

关键词: 木糖, 葡萄糖, 发酵, 燃料乙醇

Abstract: Fermentabilities of four strains, Candida shehatae NLP21, Pichia stipitis NLP22, NLP23 and NLP31 on 30 g/L xylose or mixture sugars (glucose 15 g/L and xylose 15 g/L) of the fermentation medium, and on the fermentation medium of the concentrations of the nitrogen sources reduced to 1/2 and 1/10 of its original were studied. The results showed that the mass concentrations of ethanol produced by NLP23 and NLP31 were the highest, (11.14±0.13) and (11.15±0.08) g/L respectively, when they were fermented on the fermentation medium with 30 g/L xylose. When four strains fermented on the fermentation medium with 15 g/L glucose and 15 g/L xylose, the mass concentration of ethanol produced by NLP31 was the highest, (10.91±0.12) g/L. The fermentability of NLP23 was the same as NLP31 when the concentration of nitrogen sources was reduced to 1/2 of its original, but NLP31 produced more xylitol. The fermentabilities decreased step by step with the fermentation batch increasing when the mass concentration of nitrogen sources was reduced to 1/10 of its original, because the mass concentration of the nitrogen source was too low.

Key words: xylose, glucose, fermentation, fuel ethanol

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