芒草酸/碱预处理副产物的生成及对乙醇发酵的影响

doi:10.3969/j.issn.1673-5854.2016.03.007

生物质化学工程 ›› 2016, Vol. 50 ›› Issue (3): 41-45.doi: 10.3969/j.issn.1673-5854.2016.03.007

芒草酸/碱预处理副产物的生成及对乙醇发酵的影响

佀胜利^1,2,3, 李鸣^1,2, 贾军^1,4, 李庆^1,5, 郝勃^1,2, 王艳婷^1,4, 彭良才^1,2,4, 涂媛苑^1,4

1. 华中农业大学生物质与生物能源研究中心, 湖北武汉 430070;
2. 华中农业大学生命科学技术学院, 湖北武汉 430070;
3. 凯里学院环境与生命科学学院, 贵州凯里 556011;
4. 华中农业大学植物科学技术学院, 湖北武汉 430070;
5. 华中农业大学理学院, 湖北武汉 430070

收稿日期:2015-10-13 出版日期:2016-05-31 发布日期:2016-06-03
通讯作者: 涂媛苑,女,高级工程师,研究领域为生物质与生物能源;E-mail:yuant@mail.hzau.edu.cn。 E-mail:yuant@mail.hzau.edu.cn
作者简介:佀胜利(1982-),男,山东郓城人,讲师,博士,研究方向为生物质资源与纤维乙醇转化;E-mail:shengli_si@163.com
基金资助:
教育部、国家外国专家局高等学校学科创新引智计划项目(B08032);教育部"长江学者"科研启动项目(52204-07022);中央高校基本科研业务费专项基金(2662015PY018)

Effects of Byproducts Obtained from Alkali or Acid Pretreatment of Miscanthus Biomass on Yeast Fermentation

SI Sheng-li^1,2,3, LI Ming^1,2, JIA Jun^1,4, LI Qing^1,5, HAO Bo^1,2, WANG Yan-ting^1,4, PENG Liang-cai^1,2,4, TU Yuan-yuan^1,4

1. Biomass and Bioenergy Research Centre, Huazhong Agricultural University, Wuhan 430070, China;
2. College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China;
3. College of Environment and Life Science, Kaili University, Kaili 556011, China;
4. College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China;
5. College of Science, Huazhong Agricultural University, Wuhan 430070, China

Received:2015-10-13 Online:2016-05-31 Published:2016-06-03

摘要/Abstract

摘要： 研究了不同浓度酸/碱预处理下芒草预处理上清液中副产物的生成和预处理上清液发酵产乙醇的规律,并对预处理副产物与乙醇发酵的关系进行了相关性分析。结果表明:酸/碱预处理上清液中预处理副产物浓度差异较为明显,酸处理产生的糠醛、5-羟甲基糠醛浓度明显高于碱处理,而碱处理上清液中丁香酸、香草醛、对香豆酸、阿魏酸浓度明显大于酸处理;预处理上清液补糖发酵中,1%H₂SO₄预处理上清液糖醇转化效率高于10 g/L NaOH预处理,半纤维素含量高和木质素含量低的材料具有较高的糖醇转化效率;糖醇转化效率与相应预处理上清液中副产物浓度相关性分析显示,糖醇转化效率与5-羟甲基糠醛呈极显著正相关,相关系数达0.795,与香草醛和丁香酸分别表现为极显著和显著负相关,相关系数分别为-0.811和-0.671。

关键词: 芒草, 预处理副产物, 化学预处理, 乙醇发酵, 糖醇转化效率

Abstract: Total supernatants were obtained from various alkali or acid pretreatments of Miscanthus biomass in order to detect the released byproducts and the ethanol production by yeast fermentation.And the correlations of byproducts and ethanol fermentation were analyzed.The results showed that concentrations of byproducts in supernatant obtained by different pretreatments were different.The contents of furfural and 5-hydroxymethylfurfural of acid pretreatment were higher than those of alkali pretreatment.And the contents of vanillin,p-coumaric acid,ferulic acid of alkali preteratment were higher.In the feeding glucose fermentaion of supernatant,the sugar-alcohol conversion efficiency of 1% H₂SO₄ pretreatment was higher than that of 10 g/L NaOH.The hemicelluloses and lignin contents in raw Miscanthus biomass materials respectively exhibited positive and negative effects on sugar-ethanol conversion rate.Correlative analysis indicated that vanillin and syringic acid exhibited negative effects on ethanol production,while 5-HMF was a positive factor.

Key words: Miscanthus, byproduct, chemical pretreatment, yeast fermentation, sugar-ethanol conversion rate

中图分类号:

TQ35
TK6

佀胜利, 李鸣, 贾军, 李庆, 郝勃, 王艳婷, 彭良才, 涂媛苑. 芒草酸/碱预处理副产物的生成及对乙醇发酵的影响[J]. 生物质化学工程, 2016, 50(3): 41-45.

SI Sheng-li, LI Ming, JIA Jun, LI Qing, HAO Bo, WANG Yan-ting, PENG Liang-cai, TU Yuan-yuan. Effects of Byproducts Obtained from Alkali or Acid Pretreatment of Miscanthus Biomass on Yeast Fermentation[J]. bce, 2016, 50(3): 41-45.

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芒草酸/碱预处理副产物的生成及对乙醇发酵的影响

Effects of Byproducts Obtained from Alkali or Acid Pretreatment of Miscanthus Biomass on Yeast Fermentation

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