1, 2:5,6-Di-O-isopropylidene-α-D-glucofuranose(ODG) was prepared with α-D-glucose as raw material by hydroxyl protection method. Then poly(D-lactic acid) (PDLA) coplymerized with ODG to obtain PDLAODG.Poly(D-lactic acid-co-glucose) copolymer (PDLAG) was obtainedby removing the hydroxyl protective groups of glucofuranose in PDLAODG.The structure and properties of PDLAODG and PDLAG were measured by 1H NMR spectroscopy, Fourier transform infrared spectroscopy, thermogravimetry, differential scanning calorimetry, X-ray diffraction, contact angle and water absorptionetc. The results showed that PDLAODG and PDLAG could be prepared by hydroxyl protection methodand melt coplymerization. The number of PDLA chains linking to glucose groups could be regulated by the ratio of ODG to PDLA. when the molar ratio of ODG to PDLA ≥ 5, the copolymer only contained one D-lactic acid chain. The PDLAchains of both PDLAODG and PDLAG could form α-type crystals. With the increase of glucose group content, the melting point of PDLAODG decreased from 151.5 ℃ to 147.9 ℃, the crystallinity decreased from 58.2% to 42.1%, the water contact angle decreased from 78.1° to 71.5°, the melting point of PDLAG decreased from 156.8 ℃ to 149.4 ℃, and the crystallinity decreased from 61.9% to 50.0%, the water contact angle from 70.1° to 64.4°. The hydrophilicities of PDLAODG and PDLAG were improved compared with that of neat PDLA, while the crystallinity, melting point and hydrophilicity of PDLAG were all higher than those of PDLAODG with the same glucose group content, i.e.the thermal stability, crystallization performance and hydrophilicity of PDLAG were all improved.