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生物质化学工程 ›› 2006, Vol. 40 ›› Issue (3): 9-12.

• 研究报道 • 上一篇    下一篇

大豆秸秆酶解物发酵制备l-乳酸的研究

徐忠1, 杨萍1, 赵兰天2   

  1. 1. 哈尔滨商业大学, 食品工程学院, 黑龙江 哈尔滨 150076;2. 中国人民大学, 环境学院, 北京 100782
  • 收稿日期:2006-01-04 修回日期:1900-01-01 出版日期:2006-05-30 发布日期:2006-05-30

Production of l-Lactic Acid from Soybean Straw Enzymatic Hydrolysate Fermentated by Lactobacillus Casei

XU Zhong1, YANG Ping1, ZHAO Lan-tian2   

  1. 1. School of Food Engineering, Harbin University of Commerce, Harbin 150076, China;2. Environmental Institute of Renmin University of China, Beijing 100872, China
  • Received:2006-01-04 Revised:1900-01-01 Online:2006-05-30 Published:2006-05-30

摘要: 从所选取的产l-乳酸的4株菌中筛选出一株性能优良、糖利用率和乳酸产量较高的菌种作为试验用菌种。对影响乳酸菌发酵大豆秸秆酶解物制备l-乳酸的因素进行了研究,影响大豆秸秆酶解液发酵的主要因素是菌种类型、底物糖浓度、接种量、温度及pH值。结果表明:干酪乳酸菌是较适宜的发酵菌种,较适宜的发酵条件为:发酵温度30℃,接种量10%,pH值5.5。随着底物糖含量的增加,乳酸产率相应增大,在实验范围内,乳酸菌发酵所产乳酸的产率达到80%。

关键词: l-乳酸, 干酪乳杆菌, 发酵, 大豆秸秆酶解液

Abstract: From four bacteria producing l-lactic acid, one bacterium was selected as an experimental bacterium according to its good performance, high sugar utilization ratio and lactic acid output. The factors affecting fermentated of soybean straw enzymatic hydrolysate by Lactobacillus casei had been studied. The optimum fermentation conditions were found as following: the suitable inoculating amount of Lactobacillus casei 10%, temperature 30℃, pH 5.5. With the increase of the sugar content in substrate enzymatic hydrolysate, the output of lactic acid increased correspondingly even up to 80%.

Key words: l-lactic acid, Lactobacillus casei, fermentation, soybean straw

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