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生物质化学工程 ›› 2007, Vol. 41 ›› Issue (5): 13-17.

• 研究报告 • 上一篇    下一篇

麦草酸水解及水解液木糖发酵的研究

吴真, 林鹿, 庞春生, 孙勇, 彭红, 李嘉喆   

  1. 华南理工大学 制浆造纸国家重点实验室, 广东 广州 510640
  • 收稿日期:2007-01-29 修回日期:1900-01-01 出版日期:2007-09-30 发布日期:2007-09-30
  • 通讯作者: 林 鹿,男,教授,博士生导师,主要从事植物资源化学与制浆造纸生物技术领域的研究工作;E-mail:lclulin@scut.edu.cn。

Study on Acid Hydrolysis of Wheat Straw and Fermentation of Xylose in Hydrolysate to Xylitol

WU Zhen, LIN Lu, PANG Chun-sheng, SUN Yong, PENG Hong, LI Jia-zhe   

  1. State Key Lab of Pulp and Paper Engineering, South China University of Technology, Guangzhou 510640, China
  • Received:2007-01-29 Revised:1900-01-01 Online:2007-09-30 Published:2007-09-30

摘要: 采用甲酸/盐酸水解体系水解麦草,发现 65℃ 水解 0.5 h 可实现麦草中半纤维素的充分水解。选用热带假丝酵母发酵麦草甲酸水解液制取木糖醇。分别研究了在不同浓度甲酸及甲酸根条件下D-木糖的发酵效果,发现在 2 g/L 的甲酸及 5 g/L 的甲酸根条件下,D-木糖实现发酵并得到最大产率的木糖醇,高浓度的甲酸及甲酸根都会抑制D-木糖的发酵。采用D311型阴离子交换树脂脱除甲酸根,实现了麦草水解液的发酵,木糖醇最高得率为 16.88%(木糖醇/木糖)。

关键词: 麦草水解, 甲酸, 木糖, 木糖醇, 发酵

Abstract: Hemicellulose could be hydrolyzed adequately when wheat straw was retained in the system of formic acid/hydrochloric acid for 0.5 h under 65℃. Straw hydrolysate was fermented by Candida tropicalis to produce xylitol. The effects of different concentration of formic acid and formate on the fermentation of D-xylose were studied. D-xylose could be fermented to produce xylitol availably with a concentration of 2 g/L formic acid or 5 g/L formate. A high concentration of formic acid or formate restrained the fermentation of D-xylose. Straw formic acid hydrolysate could be fermented to produce xylitol after the formate was removed by ion exchange resins of D311. All xylose was consumed within 72 h with a xylitol yield of 16.88%(xylitol/xylose).

Key words: wheat straw hydrolysis, formic acid, xylose, xylitol, fermentation

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