欢迎访问《生物质化学工程》，
生物质化学工程

生物质化学工程 ›› 2008, Vol. 42 ›› Issue (3): 17-20.

• 论文 • 上一篇    下一篇

改造盘磨机对提高喜树碱提取得率的研究

孙玉, 房桂干, 王成章, 施英乔   

  1. 中国林业科学研究院 林产化学工业研究所;国家林业局 林产化学工程重点开放性实验室, 江苏 南京 210042
  • 收稿日期:2007-11-28 修回日期:1900-01-01 出版日期:2008-05-30 发布日期:2008-05-30
  • 通讯作者: 房桂干,研究员,博士生导师,主要从事速生木材高得率制浆研究。

Research on Improving the Extraction Yield for Camptothecin by Reconstruction of Refiner

SUN Yu, FANG Gui-gan, WANG Cheng-zhang, SHI Ying-qiao   

  1. Institute of Chemical Industry of Forestry Products, CAF;Key and Open Lab.on Forest Chemical Engineering, SFA, Nanjing 210042, China
  • Received:2007-11-28 Revised:1900-01-01 Online:2008-05-30 Published:2008-05-30

PDF (PC)

1028

摘要： 采用盘磨设备从喜树果实中提取喜树碱和10-羟基喜树碱,并对盘磨机进行了技术改造,增设了不锈钢料仓和中浓泵,实现了循环进料,主轴转速由 1850 r/min 提高到 5000 r/min。比较了盘磨机改造前后从喜树果实中提取喜树碱和10-羟基喜树碱的效果,并通过正交试验进一步考察了喜树碱得率的影响因素。结果表明:盘磨设备经过改造后,喜树碱的得率相对提高 14.81%～35.29%、10-羟基喜树碱的得率相对提高 24.00%～44.44%, 喜树碱得率达到 0.06%,10-羟基喜树碱的得率达到 0.04%。 正交试验结果表明, NaOH 溶液质量分数影响最为显著。

关键词: 喜树碱, 10-羟基喜树碱, 盘磨机改造, 高效液相色谱法

Abstract: In this research refiner is used to extract camptothecin(CPT) and 10-hydroxycamptothecin(HCPT) from the fruits of Camptotheca acuminata. The refiner has been reconstructed, adding mid-level concentration feeding pump, the rotate speed of principal axis has been increased to 5000 r/min from 1850 r/min. The contents of camptothecin and 10-hydroxycamptothecin are determined by HPLC. It can be seen that the extraction ratio of camptothecin has been increased by 14.81%-35.29% and that of 10-hydroxycamptothecin has been increased by 24.00%-44.44%. The yield of CPT is 0.06% and the yield of HCPT is 0.04%. The results of orthogonal test show that the mass fraction of sodium hydroxide solution has a significant effect.

Key words: camptothecin, 10-hydroxycamptothecin, reconstruction of refiner, HPLC

中图分类号: 

版权所有 © 中国林业科学研究院林产化学工业研究所　苏ICP备08107184号-5