大孔树脂分离纯化核桃青皮总黄酮的研究

生物质化学工程 ›› 2008, Vol. 42 ›› Issue (3): 21-25.

大孔树脂分离纯化核桃青皮总黄酮的研究

翟梅枝, 郭琪, 贾彩霞, 张新华

西北农林科技大学林学院, 陕西杨凌 712100

收稿日期:2007-10-30 修回日期:1900-01-01 出版日期:2008-05-30 发布日期:2008-05-30

Study on the Separation and Purification of Flavonoids from Walnut Peel with Macroporous Resin

ZHAI Mei-zhi, GUO Qi, JIA Cai-xia, ZHANG Xin-hua

College of forest, Northwest Agriculture and Forestry of University, Yangling 712100, China

Received:2007-10-30 Revised:1900-01-01 Online:2008-05-30 Published:2008-05-30

摘要/Abstract

摘要： 以总黄酮回收率为考察指标,研究了大孔树脂分离纯化核桃青皮总黄酮的工艺。结果表明:D101型树脂对核桃青皮总黄酮有较好的吸附分离性能,是分离纯化核桃青皮总黄酮的适宜大孔树脂;最佳工艺条件为:上柱总黄酮与干树脂质量比为1:12,上样液质量浓度可在3.0875～6.175 g/L 范围内,pH值为5, 6BV(1BV=23.7 mL)的水洗后用5BV的70%乙醇洗脱。经D101处理后的核桃青皮总黄酮回收率在60%,纯度可达80%以上。该工艺简便,能有效分离纯化核桃青皮黄酮类化合物。

关键词: 核桃青皮, 总黄酮, 大孔吸附树脂, 分离纯化

Abstract: A method for separation and purification of flavonoids from walnut peel with macroporous resin was studied with the recovery rate of flavonoids as indexes. The results are as flollows: among six types of macroporous adsorption resin choosed, type macroporous resin D101 is the best for separation and purification. The optimum process condition was the loaded amount of flavonoids to dry resin: 1:12, the sample concentration:3.0875-6.175 g/L, pH:5. After eluted with 6BV of distilled water and 5BV of 70% ethanol, the yield of flavonoids was above 60% and product purity was above 80%. The process is simple and the flavonoids of walnut peel can be separated and purified effectively.

Key words: walnut peel, flavonoids, macroporous adsorption resin, separation and purification

中图分类号:

TQ91
TQ425

翟梅枝;郭琪;贾彩霞;张新华. 大孔树脂分离纯化核桃青皮总黄酮的研究[J]. 生物质化学工程, 2008, 42(3): 21-25.

ZHAI Mei-zhi;GUO Qi;JIA Cai-xia;ZHANG Xin-hua. Study on the Separation and Purification of Flavonoids from Walnut Peel with Macroporous Resin[J]. , 2008, 42(3): 21-25.

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