高效液相色谱-内标法测定焦性没食子酸含量

doi:10.3969/j.issn.1673-5854.2015.02.003

生物质化学工程 ›› 2015, Vol. 49 ›› Issue (2): 12-16.doi: 10.3969/j.issn.1673-5854.2015.02.003

高效液相色谱-内标法测定焦性没食子酸含量

张亮亮, 汪咏梅, 徐曼, 陈笳鸿

中国林业科学研究院林产化学工业研究所;生物质化学利用国家工程实验室;国家林业局林产化学工程重点开放性实验室;江苏省生物质能源与材料重点实验室, 江苏南京 210042

收稿日期:2014-07-16 出版日期:2015-03-30 发布日期:2015-04-07
通讯作者: 汪咏梅,副研究员,主要从事植物资源化学利用研究;E-mail:njlhswym@163.com。 E-mail:njlhswym@163.com
作者简介:张亮亮(1981-),男,江苏沭阳人,副研究员,博士,主要从事植物化学研究;E-mail:zhll2086@gmail.com
基金资助:
国家863计划资助(2014AA021802)

Determination of Pyrogallol Using Reversed-phase HPLC and Internal Standard Method

ZHANG Liang-liang, WANG Yong-mei, XU Man, CHEN Jia-hong

Institute of Chemical Industry of Forest Products, CAF;National Engineering Lab.for Biomass Chemical Utilization; Key and Open Lab.of Forest Chemical Engineering, SFA;Key Lab.of Biomass Energy and Material, Jiangsu Province, Nanjing 210042, China

Received:2014-07-16 Online:2015-03-30 Published:2015-04-07

摘要/Abstract

摘要： 建立了一种方便、快速、准确的测定焦性没食子酸含量的高效液相色谱-内标分析方法。色谱条件为:Thermo Hypersil C₁₈(250 mm×4.6 mm,5 μm)色谱柱,流动相为甲醇/水(含0.1%的三氟乙酸),梯度洗脱,流速为1.0 mL/min,紫外检测波长为266 nm。选择鞣花酸作为内标物。结果表明在该色谱条件下,焦性没食子酸和鞣花酸的保留时间分别为4.33和19.25 min,焦性没食子酸质量浓度在62.5~2 000 mg/L范围内呈良好线性关系,线性方程y=0.000 138 66x+0.001 92(R=0.999 7),平均加样回收率为103.6%。该方法简便、准确、重现性好,可适用于焦性没食子酸产品含量的分析测定。

关键词: 焦性没食子酸, 没食子酸, 反相高效液相色谱法, 内标法

Abstract: A rapid,specific,and accurate HPLC method was developed and validated for the assay of pyrogallol.The assay involved a gradient elution of pyrogallol with a Thermo Hypersil C₁₈ column (250 mm×4.6 mm,5 μm) using mobile phase composition of methanol and water (0.1% TFA) at a flow rate of 1.0 mL/min.UV detection was performed at 266 nm.Ellagic acid was used as an internal standard.The retention time of pyrogallol and ellagic acid was 4.33 and 19.25 min,respectively.The linearity range of pyrogallol concentration was from 62.5 to 2 000 mg/L.The fitting equation of calibration curve was y=0.000 138 66x+0.001 92 (R=0.999 7) and a mean recovery of 103.6% was found.The proposed method was applied for the quantitative determination of pyrogallol in pyrogallol products.

Key words: pyrogallol, ellagic acid, RP-HPLC, internal standard method

中图分类号:

TQ35

张亮亮, 汪咏梅, 徐曼, 陈笳鸿. 高效液相色谱-内标法测定焦性没食子酸含量[J]. 生物质化学工程, 2015, 49(2): 12-16.

ZHANG Liang-liang, WANG Yong-mei, XU Man, CHEN Jia-hong. Determination of Pyrogallol Using Reversed-phase HPLC and Internal Standard Method[J]. bce, 2015, 49(2): 12-16.

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高效液相色谱-内标法测定焦性没食子酸含量

Determination of Pyrogallol Using Reversed-phase HPLC and Internal Standard Method

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