过氧甲酸预处理对甘蔗渣酶解和发酵的影响

doi:10.3969/j.issn.1673-5854.2022.05.005

生物质化学工程 ›› 2022, Vol. 56 ›› Issue (5): 30-36.doi: 10.3969/j.issn.1673-5854.2022.05.005

过氧甲酸预处理对甘蔗渣酶解和发酵的影响

李宁¹, 孟繁阳¹, 杨海艳¹^,², 史正军¹^,², 赵平¹^,², 杨静¹^,²^,*()

1. 西南林业大学化学工程学院, 云南昆明 650224
2. 云南省高校特色林木生物质资源化学利用重点实验室, 西南林业大学, 云南昆明 650224

收稿日期:2021-08-17 出版日期:2022-09-30 发布日期:2022-09-27
通讯作者: 杨静 E-mail:kmjingyang@163.com
作者简介:杨静, 教授, 博士生导师, 研究领域: 生物质资源化学转化与利用; E-mail: kmjingyang@163.com
李宁(1996— ), 女, 内蒙古呼和浩特人, 硕士生, 主要从事生物质转化利用与研究
基金资助:
云南省基础研究计划重点项目(2019FA014);云南省中青年学术和技术带头人后备人才项目(202005AC160044);国家自然科学基金资助项目(32060329)

Effect of Peroxyformic Acid Pretreatment on Enzymatic Hydrolysis and Fermentation Efficiency of Sugarcane Bagasse

Ning LI¹, Fanyang MENG¹, Haiyan YANG¹^,², Zhengjun SHI¹^,², Ping ZHAO¹^,², Jing YANG¹^,²^,*()

1. School of Chemical Engineering, Southwest Forestry University, Kunming 650224, China
2. Key Laboratory for Chemical Utilization of Forest Biomass Resources in Colleges and Universities of Yunnan Province, Southwest Forestry University, Kunming 650224, China

Received:2021-08-17 Online:2022-09-30 Published:2022-09-27
Contact: Jing YANG E-mail:kmjingyang@163.com

摘要/Abstract

摘要：

以甘蔗渣(SCB)为原料, 经过氧甲酸(PAP)预处理后加入酶进行水解, 并以水解液发酵产乙醇, 考察预处理时过氧化氢(HPP)浓度变化对甘蔗渣酶解和乙醇得率的影响。实验结果表明: 在甘蔗渣PAP预处理过程中, HPP与甲酸(FAP)体积比为1∶1时, 预处理甘蔗渣(PAP-SCB-1)的木质素脱除率达84.30%;在纤维素酶用量为10 FPIU/g(以预处理后的甘蔗渣质量计)时, PAP-SCB-1水解72 h葡萄糖得率为98.71%, 较单独过氧化氢预处理甘蔗渣(HPP-SCB, 葡萄糖得率9.11%)和单独甲酸预处理甘蔗渣(FAP-SCB, 葡萄糖得率7.06%), 分别提高了9.84和12.98倍; PAP-SCB-1水解液经24 h发酵后, 乙醇得率为84.06%, 比HPP-SCB(76.20%)和FAP-SCB(75.15%)均有增加。对预处理前后物料的化学成分变化、比表面积和结晶度进行测定, 结果显示: 经PAP预处理后可以显著脱除甘蔗渣中的木质素, 木质素的量由未经预处理的21.27%降低到10%以下; 比表面积和结晶度都有提高, PAP-SCB-1的比表面积和结晶度分别为13.01 m²/g和54.18%, 是HPP-SCB的10.66和1.11倍, FAP-SCB的11.39和1.15倍。

关键词: 过氧甲酸, 甘蔗渣, 纤维乙醇, 酶解, 发酵

Abstract:

Sugarcane Bagasse(SCB) was used as raw material for enzymatic hydrolysis after pretreatd by peroxyformic acid(PAP). Ethanol was produced by fermentation with hydrolysate, and the effect of hydrogen peroxide(HPP) concentration on the efficiency of enzymatic hydrolysis and ethanol fermentation of SCB was investigated. The results showed that the delignification percentage of pretreated bagasse(PAP-SCB-1) reached 84.30% during PAP pretreatment of SCB when the volume ratio of HPP to formic acid(FAP) was 1∶1. Using the cellulase dosage of 10 FPIU/g(based on the mass of pretreated sugarcane bagasse), the glucose yield of PAP-SCB-1 after 72 h hydrolysis was 98.21%, which was 9.84 and 12.98 times higher than that of pretreated bagasse with hydrogen peroxide(HPP-SCB, glucose yield 9.11%) and formic acid(FAP-SCB, glucose yield 7.06%), respectively. The ethanol yield was 84.06% with the enzymatic hydrolysate fermentation for 24 h, which was higher than that of HPP-SCB(76.20%) and FAP-SCB(75.15%). The chemical composition, specific surface area and crystallinity of the materials before and after pretreatment were measured. The results showed that the lignin in bagasse could be significantly removed after pretreatment with PAP, and the lignin content was reduced from 21.27% without pretreatment to less than 10%. The specific surface area and crystallinity of PAP-SCB-1 were increased up to 13.01 m²/g and 54.18%, which were 10.66(11.39) and 1.11(1.15) times higher than those of FAP-SCB(HPP-SCB).

Key words: peroxyformic acid, sugarcane bagasse, cellulosic ethanol, enzymatic hydrolysis, fermentation

中图分类号:

TQ35

李宁, 孟繁阳, 杨海艳, 史正军, 赵平, 杨静. 过氧甲酸预处理对甘蔗渣酶解和发酵的影响[J]. 生物质化学工程, 2022, 56(5): 30-36.

Ning LI, Fanyang MENG, Haiyan YANG, Zhengjun SHI, Ping ZHAO, Jing YANG. Effect of Peroxyformic Acid Pretreatment on Enzymatic Hydrolysis and Fermentation Efficiency of Sugarcane Bagasse[J]. Biomass Chemical Engineering, 2022, 56(5): 30-36.

图/表 6

表1

图1

图2

图3

表2

图4

参考文献 22

1	吕晓静. 基于碱的木质纤维素预处理和酶解及相关机理研究[D]. 广州: 暨南大学, 2018.
2	王博伟, 闫雪晴, 何贤, 等. 纤维素可及性对木质纤维酶水解影响的研究进展[J]. 纤维素科学与技术, 2020, 28 (1): 61- 68.
3	林荣珍. 甘蔗渣综合利用发展现状探讨[J]. 企业科技与发展, 2020, (6): 62- 64. doi: 10.3969/j.issn.1674-0688.2020.06.025
4	JI Q H , YU X J , YAGOUB A E A , et al. Enhancement of lignin removal and enzymolysis of sugarcane bagasse by ultrasound-assisted ethanol synergized deep eutectic solvent pretreatment[J]. Renewable Energy, 2021, 172, 304- 316. doi: 10.1016/j.renene.2021.03.050
5	ZHAO X B , LIU D H . Fractionating pretreatment of sugarcane bagasse by aqueous formic acid with direct recycle of spent liquor to increase cellulose digestibility: The Formiline process[J]. Bioresource Technology, 2012, 117, 25- 32. doi: 10.1016/j.biortech.2012.04.062
6	WANG Q L , XIAO S L , SHI S Q , et al. Microwave-assisted formic acid extraction for high-purity cellulose production[J]. Cellulose, 2019, 26 (10): 5913- 5924. doi: 10.1007/s10570-019-02516-8
7	HAVERTY D , DUSSAN K , PITERINA A V , et al. Autothermal, single-stage, performic acid pretreatment of Miscanthus x giganteus for the rapid fractionation of its biomass components into a lignin/hemicellulose-rich liquor and a cellulase-digestible pulp[J]. Bioresource Technology, 2012, 109, 173- 177. doi: 10.1016/j.biortech.2012.01.007
8	YANG J , TU M B , XIA C L , et al. Effect of fenton pretreatment on C1 and C6 oxidation of cellulose and its enzymatic hydrolyzability[J]. ACS Sustainable Chemistry & Engineering, 2019, 7 (7): 7071- 7079.
9	BRADFORD M M . A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding[J]. Analytical Biochemistry, 1976, 72 (1/2): 248- 254.
10	应文俊, 吴凯, 史正军, 等. 磷酸联合过氧化氢预处理玉米芯及其酶水解效率的影响[J]. 林产化学与工业, 2018, 38 (5): 100- 106.
11	YANG H Y, JIN Y, SHI Z J, et al. Effect of hydrothermal pretreated bamboo lignin on cellulose saccharification for bioethanol production[J/OL]. Industrial Crops & Products, 2020, 156: 112865[2021-08-10]. https://doi.org/10.1016/j.indcrop.2020.112865.
12	李志强, 费本华, 江泽慧. 发酵抑制物对葡萄糖发酵产乙醇的影响[J]. 化工进展, 2015, 34 (增刊): 80- 84.
13	福建农林大学. 林业生物质原料分析方法不可溶性糖测定: GB/T 36058—2018[S]. 北京: 中国国家标准出版社, 2018: 12.
14	刘淑端, 张建然. 蔗渣浆纤维比表面积的测定及其对纸浆性能影响的研究[J]. 广东造纸, 1993, (2): 5- 9.
15	THYGESEN A , ODDERSHEDE J , LILHOLT H , et al. On the determination of crystallinity and cellulose content in plant fibres[J]. Cellulose, 2005, 12 (6): 563- 576. doi: 10.1007/s10570-005-9001-8
16	周浩. 木素在甲酸中的化学反应研究[D]. 济南: 齐鲁工业大学, 2020.
17	BU J, WANG Y T, DENG M C, et al. Enhanced enzymatic hydrolysis and hydrogen production of sugarcane bagasse pretreated by peroxyformic acid[J/OL]. Bioresource Technology, 2021, 326: 124751[2021-08-10]. https://doi.org/10.1016/j.biortech.2021.124751.
18	WU K , YING W J , SHI Z J , et al. Fenton reaction-oxidized bamboo lignin surface and structural modification to reduce nonproductive cellulase binding and improve enzyme digestion of cellulose[J]. ACS Sustainable Chemistry & Engineering, 2018, 6 (3): 3853- 3861.
19	MORAIS A R C , PINTO J V , NUNES D , et al. Imidazole: Prospect solvent for lignocellulosic biomass fractionation and delignification[J]. ACS Sustainable Chemistry & Engineering, 2016, 4 (3): 1643- 1652.
20	KRUYENISKI J , FERREIRA P J T , CARVALHO M D G V S , et al. Physical and chemical characteristics of pretreated slash pine sawdust influence its enzymatic hydrolysis[J]. Industrial Crops and Products, 2019, 130, 528- 536.
21	TANIGUCHI M , KOBAYASHI M , FUJⅡ M . Properties of a reversible soluble-insoluble cellulase and its application to repeated hydrolysis of crystalline cellulose[J]. Biotechnology and Bioengineering, 1989, 34 (8): 1092- 1097.
22	YANG J , ZHANG X P , YONG Q , et al. Three-stage enzymatic hydrolysis of steam-exploded corn stover at high substrate concentration[J]. Bioresource Technology, 2011, 102 (7): 4905- 4908.

样品 sample	固体回收率 solid retention rate	葡聚糖 glucan	木聚糖 xylan	木质素 lignin	纤维素回收率 recovery rate of cellulose	木质素脱除率 delignification percentage
SCB	100±0.25	46.36±0.46	22.51±1.04	21.27±0.97
HPP-SCB	92.98±1.31	47.99±1.15	21.04±1.09	19.13±0.62	96.25±1.14	10.06±0.54
FAP-SCB	92.76±1.54	47.81±1.02	20.86±1.07	18.60±0.44	95.66±1.58	12.55±1.25
PAP-SCB-1	84.87±1.93	53.73±1.06	22.52±1.01	3.34±0.64	98.36±1.96	84.30±1.56
PAP-SCB-4	84.86±1.88	52.20±0.95	21.85±0.98	3.89±0.59	95.50±2.12	81.71±1.78
PAP-SCB-8	84.58±2.03	51.58±0.89	20.88±1.06	8.40±0.48	94.10±1.22	60.51±1.45

样品 sample	乙醇质量浓度/(g·L^-1) mass concentration of ethanol	乙醇得率/% ethanol yield
SCB	2.58±0.27	68.82
HPP-SCB	2.95±0.44	76.20
FAP-SCB	2.81±0.56	75.15
PAP-SCB-1	17.10±0.45	84.06
PAP-SCB-4	13.46±0.26	81.31
PAP-SCB-8	10.29±0.34	78.37

[1]	吴松. 发酵性丝孢酵母处理精炼大豆油废水的工艺优化及动力学分析[J]. 生物质化学工程, 2022, 56(5): 51-57.
[2]	刘仕勋, 刘豪杰, 周杰, 徐宁, 信丰学, 董维亮, 姜岷. 利用废弃碳资源生产鼠李糖脂的研究进展[J]. 生物质化学工程, 2022, 56(5): 63-71.
[3]	孟繁阳, 李宁, 史正军, 杨海艳, 赵平, 杨静. 过氧化氢-乙酸联合预处理对甘蔗渣酶解和发酵的影响[J]. 生物质化学工程, 2022, 56(4): 25-31.
[4]	李希越, 王洪波, 赵玉晓, 华栋梁, 王宁. 好氧生物预处理时间对玉米秸秆水解酸化的影响[J]. 生物质化学工程, 2022, 56(1): 13-22.
[5]	阚玉娜, 陈冰炜, 翟胜丞, 潘明珠, 梅长彤. 有机溶剂自催化和协同预处理促进木质纤维原料酶解研究进展[J]. 生物质化学工程, 2020, 54(6): 74-82.
[6]	杨浩,陈琪,赵慧敏,顾浩杰,阎杰,林海琳. 甘蔗渣综纤维素膜的制备和性能研究[J]. 生物质化学工程, 2020, 54(2): 6-14.
[7]	李冬娜,马晓军. 污泥厌氧发酵产酸机理及应用研究进展[J]. 生物质化学工程, 2020, 54(2): 51-60.
[8]	刘科,贺静,韦秀丽,蒋滔,唐宁,张德勇. 水稻秸秆渗滤床半固态厌氧发酵性能研究[J]. 生物质化学工程, 2020, 54(1): 1-8.
[9]	刘雪梅,马闯,陶嘉熙. 草酸改性甘蔗渣炭的制备及其对Cr(Ⅵ)的吸附特性[J]. 生物质化学工程, 2019, 53(4): 37-44.
[10]	郭腾飞, 高士帅, 王春鹏. 酶解木质素改性三聚氰胺脲醛树脂的制备与应用[J]. 生物质化学工程, 2018, 52(3): 35-39.
[11]	魏薇, 常福祥, 孙建中, 王钱钱. 木质纤维“一锅法”制备生物乙醇的研究进展[J]. 生物质化学工程, 2018, 52(1): 53-59.
[12]	邢靖晨, 于丽丽, 马晓军. 加压热水预处理温度对毛白杨木材结构及酶解效果的影响[J]. 生物质化学工程, 2017, 51(5): 36-40.
[13]	单增宇, 马灼明, 李淑君. 酶解木质素的碱催化温和水热降解[J]. 生物质化学工程, 2017, 51(2): 8-12.
[14]	张宪宝, 张腾, 谢文化, 朱明军. 氢氧化钠预处理对甘蔗渣酶解和发酵性能的影响[J]. 生物质化学工程, 2016, 50(6): 9-16.
[15]	华洋林, 张苗, 李俊, 王群, 陈彤, 唐健, 曹庸. 灵芝提取液的发酵优化及保湿性能评价[J]. 生物质化学工程, 2016, 50(5): 22-28.

过氧甲酸预处理对甘蔗渣酶解和发酵的影响

Effect of Peroxyformic Acid Pretreatment on Enzymatic Hydrolysis and Fermentation Efficiency of Sugarcane Bagasse

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

图/表 6

参考文献 22

相关文章 15

编辑推荐

Metrics

本文评价

关于本刊

投稿指南

在线期刊

相关单位

联系我们