过氧化氢-乙酸联合预处理对甘蔗渣酶解和发酵的影响

doi:10.3969/j.issn.1673-5854.2022.04.004

生物质化学工程 ›› 2022, Vol. 56 ›› Issue (4): 25-31.doi: 10.3969/j.issn.1673-5854.2022.04.004

过氧化氢-乙酸联合预处理对甘蔗渣酶解和发酵的影响

孟繁阳¹(), 李宁¹, 史正军¹^,², 杨海艳¹^,², 赵平¹^,², 杨静¹^,²^,*()

1. 西南林业大学化学工程学院, 云南昆明 650224
2. 云南省高校特色林木生物质资源化学利用重点实验室; 西南林业大学, 云南昆明 650224

收稿日期:2021-08-20 出版日期:2022-07-30 发布日期:2022-07-23
通讯作者: 杨静 E-mail:603294704@qq.com;kmjingyang@163.com
作者简介:杨静, 教授, 博士生导师, 研究领域: 生物质资源化学转化与利用; E-mail: kmjingyang@163.com
孟繁阳(1995-), 男, 河南许昌人, 硕士生, 研究方向为生物质转化和利用; E-mail: 603294704@qq.com
基金资助:
云南省基础研究计划重点项目(2019FA014);国家自然科学基金资助项目(31760194);国家自然科学基金资助项目(32060329)

Effect of Hydrogen Peroxide-acetic Acid Pretreatment on Enzymatic Hydrolysis and Fermentation of Sugarcane Bagasse

Fanyang MENG¹(), Ning LI¹, Zhengjun SHI¹^,², Haiyan YANG¹^,², Ping ZHAO¹^,², Jing YANG¹^,²^,*()

1. School of Chemical Engineering, Southwest Forestry University, Kunming 650224, China
2. Key Laboratory for Chemical Utilization of Forest Biomass Resources in Colleges and Universities of Yunnan Province, Southwest Forestry University, Kunming 650224, China

Received:2021-08-20 Online:2022-07-30 Published:2022-07-23
Contact: Jing YANG E-mail:603294704@qq.com;kmjingyang@163.com

摘要/Abstract

摘要：

采用过氧化氢-乙酸(HPAC)对甘蔗渣(SCB)进行了联合预处理。以预处理后的甘蔗渣为原料, 先进行酶水解, 然后将水解液进行乙醇发酵, 探讨预处理对甘蔗渣酶解和发酵的影响。实验结果表明: 20 g甘蔗渣, 加入150 mL过氧化氢水溶液(75 mL过氧化氢(30%)和75 mL水)和150 mL乙酸(99%), 硫酸用量为过氧化氢-乙酸溶液体积的0.5%, 在70 ℃反应2 h时, HPAC预处理脱除了88.85%的木质素, 并使90.10%的纤维素保留在底物中。底物(HPAC/70-SCB-0.5)的酶可及度是80.30 mg/g, 与相同条件下单独过氧化氢预处理(HP/70-SCB)和单独乙酸预处理(AC/70-SCB)相比, 分别增加了38.26%和31.08%, 甘蔗渣木质素的表面覆盖率从原料的0.66降低至0.22。酶解上清液在酶用量为5 FPIU/g(以底物计)条件下水解后, 葡萄糖得率是87.63%, 分别是HP/70-SCB和AC/70-SCB的6.89和20.62倍, 发酵产乙醇质量浓度是7.57 g/L, 分别是HP/70-SCB和AC/70-SCB的7.65和22.94倍。

关键词: 甘蔗渣, 过氧化氢-乙酸联合预处理, 脱木素, 酶水解, 发酵

Abstract:

Sugarcane bagasse(SCB) was pretreated by hydrogen peroxide-acetic acid(HPAC). The pretreated SCB was used as raw material for enzymatic hydrolysis, and then the hydrolysate was fermented for ethanol. The effects of pretreatment on enzymatic hydrolysis and fermentation of SCB were discussed. The results showed that HPAC pretreatment removed 88.85% lignin and retained 90.10% cellulose in the substrate when 20 g SCB was added with 150 mL aqueous hydrogen peroxide(i.e., 75 mL hydrogen peroxide(30%) and 75 mL water) and 150 mL acetic acid(99%), the amount of sulfuric acid was 0.5% of the HPAC solution volume, and the reaction was preformed at 70 ℃ for 2 h. The enzyme accessibility of substrate was 80.30 mg/g, which increased by 38.26% and 31.08% compared with those of hydrogen peroxide pretreatment (HP/70-SCB) and acetic acid pretreatment(AC/70-SCB) under the same conditions, respectively, and the surface coverage of lignin decreased from 0.66 to 0.22. After hydrolysis with enzyme dosage of 5 FPIU/g(substrate), the yield of glucose was 87.63%, which was 6.89 and 20.62 times than those of HP/70-SCB and AC/70-SCB, respectively. The mass concentration of ethanol produced by fermentation was 7.57 g/L, which was 7.65 and 22.94 times than those of HP/70-SCB and AC/70-SCB, respectively.

Key words: sugarcane bagasse, HPAC pretreatment, delignification, enzymatic hydrolysis, fermentation

中图分类号:

TQ35

孟繁阳, 李宁, 史正军, 杨海艳, 赵平, 杨静. 过氧化氢-乙酸联合预处理对甘蔗渣酶解和发酵的影响[J]. 生物质化学工程, 2022, 56(4): 25-31.

Fanyang MENG, Ning LI, Zhengjun SHI, Haiyan YANG, Ping ZHAO, Jing YANG. Effect of Hydrogen Peroxide-acetic Acid Pretreatment on Enzymatic Hydrolysis and Fermentation of Sugarcane Bagasse[J]. Biomass Chemical Engineering, 2022, 56(4): 25-31.

图/表 6

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参考文献 22

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样品¹⁾ sample	回收率 solid residue yield	葡聚糖 glucan	木聚糖 xylan	木质素 lignin	纤维素回收率 recovery of cellulose	木质素脱除率 delignification
SCB	100±0.11	49.02±1.01	22.78±1.33	24.38±0.62
HP/70-SCB	98.00±0.73	49.70±0.92	21.52±1.22	23.57±1.66	99.35±1.03	5.28±0.23
AC/70-SCB	93.36±1.21	48.79±1.33	22.61±0.97	20.65±1.31	92.92±2.34	17.45±1.00
HPAC/30-SCB	87.70±2.01	53.61±1.13	24.71±0.85	17.58±1.56	95.91±0.98	30.27±0.97
HPAC/50-SCB	83.93±1.85	58.39±0.97	25.24±0.77	9.76±1.75	99.97±2.22	66.42±1.56
HPAC/70-SCB	60.40±1.63	74.18±0.72	21.23±0.22	2.58±0.13	91.40±2.31	95.30±0.93
HPAC/70-SCB-0.5	70.40±2.24	62.74±0.56	25.57±1.54	4.50±0.22	90.10±1.78	88.85±1.60

样品 sample	氧碳比 ratio of oxygen to carbon	表面覆盖率/% surface coverage		C—C/C—H/% (284.6 eV)	C—O/% (286.6 eV)	C＝O/% (287.6 eV)	O—C＝O/% (289 eV)
样品 sample	氧碳比 ratio of oxygen to carbon	S_L	S_C	C—C/C—H/% (284.6 eV)	C—O/% (286.6 eV)	C＝O/% (287.6 eV)	O—C＝O/% (289 eV)
SCB	0.50	66	34	55.28	31.45	6.48	6.79
HP/70-SCB	0.67	32	68	37.13	44.82	11.87	6.17
AC/70-SCB	0.50	66	34	52.50	28.76	8.52	10.21
HPAC/70-SCB-0.5	0.72	22	78	34.86	31.05	16.36	17.73

样品 sample	疏水度/(L·g^-1) hydrophobicity	酶可及度/(mg·g^-1) enzymatic accessibility
SCB	0.173±0.004	63.34±1.22
HP/70-SCB	0.159±0.009	58.08±2.54
AC/70-SCB	0.169±0.011	61.26±3.87
HPAC/30-SCB	0.155±0.014	66.49±3.66
HPAC/50-SCB	0.085±0.016	67.39±1.98
HPAC/70-SCB	0.067±0.005	84.54±3.99
HPAC/70-SCB-0.5	0.067±0.007	80.30±4.12

样品 sample	乙醇质量浓度/(g·L^-1) ethanol mass concentration	乙醇得率/% ethanol yield
SCB	—	—
HP/70-SCB	0.99±0.11	62.55±2.01
AC/70-SCB	0.33±0.23	63.02±1.23
HPAC/30-SCB	1.29±0.20	59.26±0.99
HPAC/50-SCB	5.42±0.21	63.76±2.10
HPAC/70-SCB	6.67±0.31	57.09±2.11
HPAC/70-SCB-0.5	7.57±0.19	63.11±1.78

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过氧化氢-乙酸联合预处理对甘蔗渣酶解和发酵的影响

Effect of Hydrogen Peroxide-acetic Acid Pretreatment on Enzymatic Hydrolysis and Fermentation of Sugarcane Bagasse

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