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生物质化学工程 ›› 2012, Vol. 46 ›› Issue (5): 25-28.

• 研究报告 • 上一篇    下一篇

杂交楝果实含油量及籽油脂肪酸组成分析

朱春玲1, 周卫兵2, 何祯祥3   

  1. 1. 上海林业总站, 上海市 200072;2. 九康生物科技发展有限责任公司, 江苏 南京 210005;3. 南京大学 生命科学学院, 江苏 南京 210093
  • 收稿日期:2012-05-17 修回日期:1900-01-01 出版日期:2012-09-30 发布日期:2012-09-30
  • 通讯作者: 何祯祥,硕士生导师,目前主要从事生物农药和木本油料植物资源研发;E-mail:zxhe@nju.edu.cn。

Analysis of Fruit Oil Content and Fatty Acid Composition of Seed Kernel Oil in Azadirachta indica×Melia azedarach

ZHU Chun-ling1, ZHOU Wei-bing2, HE Zhen-xiang3   

  1. 1. Shanghai Forest Station, Shanghai 200072, China;2. Jiukang Biotechnology Development Co., Ltd., Ltd., Nanjing 210005, China;3. College of Life sciences, Nanjing University, Nanjing 210093, China
  • Received:2012-05-17 Revised:1900-01-01 Online:2012-09-30 Published:2012-09-30

摘要: 对印楝和苦楝2个亲本采用体细胞融合技术杂交形成的7年生杂交楝进行了果实含油量测定,并对其籽油进行了脂肪酸组成分析。结果表明:果皮和果肉含油量较低,分别为 1.65% 和 1.53%;种仁含油量较高,为 39.20%。种仁经提取获得籽油后进行甲酯化处理,再进行GC-MS分析,共检测出6种脂肪酸。它们是亚油酸(C18:2)67.00%、油酸(C18:1)18.03%、棕榈酸(C16:0)8.96%、硬脂酸(C18:0)3.94%、花生酸(C20:0)0.35% 和未知脂肪酸 1.72%;其中不饱和脂肪酸占 85.03%。杂交楝种仁含油量比苦楝高出1.6个百分点。

关键词: 印楝, 苦楝, 体细胞杂交, 脂肪酸

Abstract: The fruit oil content of 7-years-old hybrid neem tree which was produced from Azadirachta indica A. Juss and Melia azedarach by somatic hybridization technology, and the fatty acid composition of seed kernel oil were analyzed. Results showed lower oil content in fruit peel and fruit pulp, i.e. 1.65% and 1.53%, respectively. However, high oil content (39.20%) in seed kernel was found. Seed kernel oil was analyzed by GC-MS and six kinds of fatty acids were determined. There were linoleic acid(C18:2)67.00%, oleic acid(C18:1)18.03%, palmitic acid (C16:0)8.96%, stearic acid (C18:0)3.94%, arachidic acid (C20:0)0.35% and unknown fatty acid 1.72%. The content of unsaturated fatty acid of seed kernel oil was 85.03%. Compared with the M.azedarach, the oil content of hybrid neem tree seed kernel was 1.6 percentage points higher.

Key words: Azadirachta indica A. Juss, Melia azedarach, somatic hybridization, fatty acid

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