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生物质化学工程

生物质化学工程 ›› 2015, Vol. 49 ›› Issue (3): 1-6.doi: 10.3969/j.issn.1673-5854.2015.03.001

• 研究报告 •    下一篇

基于纤维素模板的聚合物空心微球作为药物载体的性能研究及分析

南静娅1, 刘玉鹏1, 陈莹1,2, 王春鹏1,2, 储富祥3   

  1. 1. 中国林业科学研究院 林产化学工业研究所; 生物质化学利用国家工程实验室; 国家林业局 林产化学工程重点开放性实验室; 江苏省 生物质能源与材料重点实验室, 江苏 南京 210042;
    2. 中国林业科学研究院 林业新技术研究所, 北京 100091;
    3. 中国林业科学研究院, 北京 100091
  • 收稿日期:2014-11-13 出版日期:2015-05-30 发布日期:2015-08-04
  • 通讯作者: 王春鹏,研究员,主要从事生物质高分子材料、高分子乳液、环保型人造板胶黏剂等研究;E-mail:wangcpg@163.com。 E-mail:wangcpg@163.com
  • 作者简介:南静娅(1988—),女,山西运城人,硕士,主要从事高分子复合微球方面的研究工作
  • 基金资助:
    中国林科院林业新技术所基本科研业务费专项资金(CAFINT2010K04);江苏省自然科学基金项目(BK20131071)

Properties of Cellulose-based Polymeric Hollow Microspheres as Drug Carriers

NAN Jing-ya1, LIU Yu-peng1, CHEN Ying1,2, WANG Chun-peng1,2, CHU Fu-xiang3   

  1. 1. Institute of Chemical Industry of Forest Products, CAF; National Engineering Lab.for Biomass Chemical Utilization; Key and Open Lab.of Forest Chemical Engineering, SFA; Key Lab.of Biomass Energy and Material, Jiangsu Province, Nanjing 210042, China;
    2. Research Institute of Forestry New Technology, CAF, Beijing 100091, China;
    3. Chinese Academy of Forestry, Beijing 100091, China
  • Received:2014-11-13 Online:2015-05-30 Published:2015-08-04

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摘要： 以羟丙基纤维素为模板材料,分别采用不同的聚合方法制备了2种不同形态和结构的聚合物空心微球——聚N-异丙基丙烯酰胺-co-聚丙烯酸(PNIPAm-co-PAA)微凝胶和聚N-异丙基丙烯酰胺-聚丙烯酸(PNIPAm-PAA)水凝胶微囊。以盐酸阿霉素(Dox)作为模型药物,考察了聚合物空心微球作为药物载体的载药能力和体外释放性能。研究表明,PNIPAm-co-PAA微凝胶、PNIPAm-PAA水凝胶微囊和Dox分子能够通过正负电荷的相互吸引实现有效结合;载药微球具有良好的缓释性能,并对Dox的释放表现出明显的pH值敏感性和温度敏感性。体外细胞毒性实验表明,载药PNIPAm-co-PAA微凝胶、PNIPAm-PAA水凝胶微囊具有很高的抗肿瘤活性,细胞相对存活率均可达20%左右。PNIPAm-co-PAA微凝胶、PNIPAm-PAA水凝胶微囊在作为水溶性药物或蛋白类药物载体方面,具有潜在的应用价值,同时有望应用于木材胶黏剂防腐等。

关键词: 聚合物空心微球, 聚N-异丙基丙烯酰胺, 聚丙烯酸, 药物载体

Abstract: Hydroxypropylcellulose (HPC), a natural polymer, was chosen as a biocompatible and biodegradable template to prepare two kinds of polymeric hollow microspheres, i.e., poly(N-isopropylacrylamide)-co-poly(acrylic acid) (PNIPAm-co-PAA) microgels and poly(N-isopropylacrylamide)-poly(acrylic acid) (PNIPAm-PAA) hydrogel capsules by using different polymerization methods.The drug loading capacity and releasing behaviors of PNIPAm-co-PAA microgels and PNIPAm-PAA hydrogel capsules as drug carriers were investigated by using anticancer drug, Doxorubicin hydrochloride (Dox), as hydrophilic model drug.The results indicated that Dox could be encapsulated in PNIPAm-co-PAA microgels and PNIPAm-PAA hydrogel capsules with high drug loading driven by the electrostatic interaction.The characteristic sustained-releases of Dox from these two kinds of microspheres were observed under physiological pH and temperature.More encouragingly, the release of Dox exhibited a dual-responsivity to temperature and pH.In vitro cytotoxicity assay indicated that Dox-loaded PNIPAm-co-PAA microgels and PNIPAm-PAA hydrogel capsules had high antitumor activity, and implied that they might be a potential drug delivery carrier especially for water-soluble or polypeptide drugs, as well as applying in the wood adhesive preservation, etc.

Key words: polymeric hollow microspheres, poly (N-isopropylacrylamide), poly (acrylic acid), drug carrier

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