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生物质化学工程

生物质化学工程 ›› 2017, Vol. 51 ›› Issue (6): 33-37.doi: 10.3969/j.issn.1673-5854.2017.06.006

• 研究报告 • 上一篇    下一篇

菊芋渣选择性吸附天然菊粉酶催化制备短链低聚果糖

李鑫1,2, 周瑾2, 赖晨欢1,2, 勇强1,2   

  1. 1. 南京林业大学 江苏省林业资源高效加工利用协同创新中心, 江苏 南京 210037;
    2. 南京林业大学 化学工程学院, 江苏 南京 210037
  • 收稿日期:2017-10-16 出版日期:2017-11-30 发布日期:2017-12-08
  • 作者简介:李鑫(1975-),男,辽宁大连人,副教授,博士,主要从事生物质资源生物利用研究工作;E-mail:xli@njfu.edu.cn。
  • 基金资助:
    江苏省重点研发计划项目（BF2015007）；青海省重点研发与转化计划项目（2016-HZ-819）；江苏省高校优势学科建设工程资助项目（无编号）

Selective Adsorption of Native Inulinase by Jerusalem Artichoke Residue and Preparation of Short-chain Fructooligosaccharides

LI Xin1,2, ZHOU Jin2, LAI Chenhuan1,2, YONG Qiang1,2   

  1. 1. Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing 210037, China;
    2. College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China
  • Received:2017-10-16 Online:2017-11-30 Published:2017-12-08

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摘要： 以菊粉加工废弃物（菊芋渣）选择性吸附黑曲霉产的天然菊粉酶提高菊粉酶活力（I）与转化酶活力（S）的比值（即I/S比值），并将此纯化后的菊粉酶应用于后续的短链低聚果糖（FOSs）的生产，考察了纯化后菊粉酶对FOSs产率的影响。结果显示：选择的较佳吸附条件为：菊芋渣质量浓度为100 g/L，吸附pH值为5.0，吸附温度为4℃。经菊芋渣的选择性吸附，天然菊粉酶酶系中转化酶活力降低80.84%，菊粉酶活力保留67.92%，I/S比值由原来的1.13上升至4.02。经菊芋渣选择性吸附后的天然菊粉酶用于制备短链低聚果糖，可显著提高短链低聚果糖的浓度，酶解条件为：菊粉质量浓度为40 g/L，酶解pH值为5.0，温度为50℃，酶用量为20 U/g （以菊粉质量计），短链低聚果糖质量浓度达到16.06g/L，是原酶液制备短链低聚果糖的2.55倍；短链低聚果糖得率为40.16%。

关键词: 菊粉酶, 选择性吸附, 菊芋渣, 菊粉, 短链低聚果糖

Abstract: A selective adsorption method was developed to adsorb native inulinase from Aspergillus niger to reduce sucrase activity by Jerusalem artichoke residue. The inulinase activity(I)/sucrase activity(S) ratio was generally used to characterize the inulinases. Afterward, the native inulinase, selectively adsorbed by Jerusalem artichoke residue, was applied for production of short-chain fructooligosaccharides(FOSs). The results showed that the optimal conditions of the selective adsorption were as follows:Jerusalem artichoke residue loading 100 g/L, pH value 5.0, adsorption temperature 4℃. 67.92% Inulinase activity was retained and 80.84% sucrase activity was decreased in the native inulinase system after the selective adsorption. The I/S ratio increased from 1.13 to 4.02. The selective adsorption of native inulinase by Jerusalem artichoke residue improved the production of short-chain FOSs. The conditions of enzymatic hydrolysis were inulin 40 g/L, pH value 5.0, 50℃, inulinase loading 20 U/g(based on the mass of inulin). The mass concentration of short-chain FOSs was 16.06 g/L, which was 2.55 times than that of the original inulinase. The yield of short-chain FOSs was 40.16%.

Key words: inulinase, selective adsorption, Jerusalem artichoke residue, inulin, short-chain fructooligosaccharides

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