欢迎访问《生物质化学工程》,

生物质化学工程 ›› 2017, Vol. 51 ›› Issue (6): 26-32.doi: 10.3969/j.issn.1673-5854.2017.06.005

• 研究报告 • 上一篇    下一篇

油菜秸秆高温水解液中单糖组分的高效液相色谱测定

冯广荣1,2, 鲁厚芳1,2, 梁斌1,2, 刘颖颖2   

  1. 1. 四川大学 化学工程学院, 四川 成都 610065;
    2. 四川大学 新能源与低碳技术研究院, 四川 成都 610065
  • 收稿日期:2016-10-27 出版日期:2017-11-30 发布日期:2017-12-08
  • 通讯作者: 刘颖颖,助理研究员,博士,研究方向为生物质能的开发;E-mail:liuyingying@scu.edu.cn。 E-mail:liuyingying@scu.edu.cn
  • 作者简介:冯广荣(1988-),女,河南安阳人,硕士生,主要从事生物质水解方法及其水解液组成的研究
  • 基金资助:
    国家自然科学基金资助项目(21336008)

Determination of Monosaccharides in High Temperature Hydrolysate of Rape Straw by High Performance Liquid Chromatography

FENG Guangrong1,2, LU Houfang1,2, LIANG Bin1,2, LIU Yingying2   

  1. 1. College of Chemical Engineering, Sichuan University, Chengdu 610065, China;
    2. Institute of New Energy and Low Carbon Technology, Chengdu 610065, China
  • Received:2016-10-27 Online:2017-11-30 Published:2017-12-08

摘要: 建立了高温水中CO2催化油菜秸秆水解体系中单糖的高效液相色谱(HPLC)分析的方法。其分析采用PrevailTM ES糖柱,蒸发光散射检测器(ELSD),流动相为乙腈/水(体积比80∶20),等度洗脱,流速0.8 mL/min,柱温35 ℃,漂移管温度95 ℃,干燥空气作为载气,流速为3.0 L/min。20 min内阿拉伯糖、木糖、果糖、甘露糖、半乳糖和葡萄糖6种单糖得到了较好的分离,各物质线性回归方程的相关系数R2为0.999 1~0.999 7,其平均回收率为95.3 %~108.0 %,相对标准偏差(RSD)均小于3 %。

关键词: 高效液相色谱, 蒸发光散射检测器, 秸秆水解液, 单糖

Abstract: The method was developed to determine monosaccharides in the high temperature hydrolysate of rape straw catalyzed by CO2 via high performance liquid chromatography(HPLC). The monosaccharides were separated on the PrevailTM ES carbohydrate column at 35 ℃ with the mixture of acetonitrile/water(80:20,volume ratio) as mobile phase, which was under isocratic elution at the flow rate of 0.8 mL/min. They were detected by evaporative light-scattering detector(ELSD) using dry air as carrier gas at flow rate of 3.0 L/min and drift tube working at 95 ℃. The HPLC analysis method could ensure six monosaccharides(including arabinose, xylose, fructose, mannose, galactose and glucose) separate well within 20 min. The resultant correlation coefficients(R2) of monosaccharides were 0.999 1-0.999 7. The average recovery values were 95.3 %-108.0 %, and the relative standard deviation percentage(RSD) was less than 3 %.

Key words: high performance liquid chromatography, evaporative light-scattering detector, straw hydrolysate, monosaccharide

中图分类号: