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生物质化学工程

生物质化学工程 ›› 2010, Vol. 44 ›› Issue (5): 35-40.

• 论文 • 上一篇    下一篇

乙醇发酵菌种的选育及发酵条件的初步探索试验

曾凡洲, 卫民, 蒋剑春, 赵剑, 李科   

  1. 中国林业科学研究院, 林产化学工业研究所;生物质化学利用国家工程实验室;国家林业局, 林产化学工程重点开放性实验室, 江苏 南京 210042
  • 收稿日期:2010-06-13 修回日期:1900-01-01 出版日期:2010-09-30 发布日期:2010-09-30
  • 通讯作者: 卫 民(1961- ),男,安徽来安人,副研究员,硕士生导师,从事木质素化学利用、植物纤维水解及树脂研究开发工作;联系电话:025-85482436;E-mail:nanjingwm@163.com。

Strain Screening of Hydrolysate Fermentation for Ethanol and Preliminary Study on Fermentation

ZENG Fan-zhou, WEI Min, JIANG Jian-chun, ZHAO Jian, LI Ke   

  1. Institute of Chemical Industry of Forest Products, CAF;National Engineering Lab. for Biomass Chemical Utilization;Key and Open Lab. on Forest Chemical Engineering, SFA, Nanjing 210042, China
  • Received:2010-06-13 Revised:1900-01-01 Online:2010-09-30 Published:2010-09-30

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摘要： 对5种燃料乙醇发酵菌种进行了选择,对较好菌种做了耐高温驯化及发酵条件的优化。结果表明,在以葡萄糖为单一碳源的发酵中,相对于休哈塔假丝酵母、嗜单宁管囊酵母、间型脉胞菌、好食脉孢菌,酿酒酵母种液制备时间最短(对数生长期在6～13 h),发酵制取乙醇得率最高;对酿酒酵母驯化后,得到一株可在42℃正常生长的菌株;经研究,该耐高温菌株的较优发酵条件为温度30℃、转速150 r/min、初始pH值5.0、接种量10%。该耐高温驯化后的酿酒酵母不能直接用于水解液发酵制乙醇,还需要经过驯化或菌种改良后方能使用。

关键词: 燃料乙醇, 水解液, 耐高温酿酒酵母, 发酵条件

Abstract: Strains were selected from five fuel ethanol hydrolysate fermentation. Thermostable domestication and the fermentation optimization of better strain were studied. The results showed that in the fermentation comparison of Candida shechatae, Pachysolen tannophilus, Neurospora intermedia and Neurospora sitophila with glucose as the single carbon source, Saccharomyces cerevisiae was the best for the highest ethanol yield and the shortest log phase with 6-13 h. By domestication, a thermostable strain was screened out, which performed normal growth under 42℃. By optimizing, the optimum fermentation conditions were determined for temperature 30℃, stirring rate 150 r/min, initial pH value 5.0, inoculation amount 10%. The yeast can not be directly used for ethanol fermentation hydrolysis. But it can be used after domesticated or strain improvement.

Key words: fuel ethanol, hydrolysate, thermostable S.cerevisiae, fermentation conditions

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