杨木纤维细胞与薄壁细胞酶解性能差异性研究

doi:10.3969/j.issn.1673-5854.2023.02.003

摘要/Abstract

摘要：

以杨木为原料, 经冰醋酸-过氧化氢处理得到杨木纤维细胞和薄壁细胞, 加入纤维素酶进行酶水解, 考察了纤维细胞与薄壁细胞酶解性能的差异性, 并对酶水解过程的影响因素、水分分布状态规律和力学性能进行分析。研究结果表明: 薄壁细胞具有更高酶促反应可及性, 酶解72 h可发酵糖达到208.6 g/L, 较纤维细胞提高8.5%。在相同木糖得率(59%)下, 薄壁细胞酶用量比纤维细胞降低了50%;在高固体系(固形物的量大于15 g/mL)下, 薄壁细胞的酶解率提高了15.4%~33.3%。水分分布状态表明, 薄壁细胞在酶解过程中水束缚作用更弱, 具有更好的液化性能。根据力学性能分析发现酶水解过程的前24 h是决定纤维细胞与薄壁细胞酶解性能差异的关键阶段。此外, 薄壁细胞在酶解过程中力学性能较弱, 有助于节约能耗和缩短转化周期。

关键词: 纤维细胞, 薄壁细胞, 酶水解, 水分分布, 力学性能

Abstract:

In this study, fiber cells and parenchyma cells of poplar were prepared from poplar by glacial acetic acid-hydrogen peroxide treatment. On this basis, the differences of enzymatic hydrolysis between fiber cells and parenchyma cells were investigated, and the influencing factors, water distribution and mechanical properties of the enzymatic hydrolysis process were analyzed. The results showed that parenchyma cells had higher accessibility of enzymatic reaction and the fermentable sugar content of parenchyma cells reached 208.6 g/L after 72 h of enzymatic hydrolysis, which was 8.5% higher than that of fibrous cells. With the same enzymatic hydrolysis efficiency of xylose(59%), the enzyme loading of parenchyma cells decreased by 50% compared to fibrous cells. The enzymatic hydrolysis rate of parenchyma cells increased by 15.4%-33.3% with the high solid system(the solid loading was more than 15 g/mL). The state of water distribution showed that parenchyma cells had weaker water binding effect during enzymatic hydrolysis process and had better liquefaction performance. The mechanical properties showed that the key stage determining the difference of enzymatic hydrolysis performance between fiber cells and parenchyma cells was the first 24 h. In addition, parenchyma cells had weak mechanical properties during enzymatic hydrolysis, which helped to save energy and shorten cycle time.

Key words: fiber cells, parenchymal cells, enzymatic hydrolysis, water distribution, mechanical property

中图分类号:

TQ35

李名路, 王岚, 陈洪章. 杨木纤维细胞与薄壁细胞酶解性能差异性研究[J]. 生物质化学工程, 2023, 57(2): 21-28.

Minglu LI, Lan WANG, Hongzhang CHEN. The Difference of Enzymatic Hydrolysis Performance Between Poplar Fiber Cells and Parenchyma Cells[J]. Biomass Chemical Engineering, 2023, 57(2): 21-28.

图/表 6

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参考文献 32

1	LIU M R , WANG L , SI M Y , et al. New insight into enzymatic hydrolysis of the rice straw and poplar: An in-depth statistical analysis on the multiscale recalcitrance[J]. BioEnergy Research, 2019, 12 (1): 21- 33. doi: 10.1007/s12155-019-9959-y
2	王东, 彭立民, 傅峰, 等. 腐朽木材的吸声性能[J]. 林业科学, 2015, 51 (11): 91- 96.
3	ARZOLA-VILLEGAS X , LAKES R , PLAZA N Z , et al. Wood moisture-induced swelling at the cellular scale-ab intra[J]. Forests, 2019, 10 (11): 996- 1015. doi: 10.3390/f10110996
4	曾薇, 陈洪章. 8种真菌对玉米秸秆细胞选择性降解的研究[J]. 生物质化学工程, 2008, 42 (5): 9- 14. doi: 10.3969/j.issn.1673-5854.2008.05.003
5	翟华敏, 李忠正. 麦草纤维细胞和薄壁细胞制浆特性的研究[J]. 中国造纸, 1989, 8 (5): 11- 15.
6	翟华敏, 李忠正. 麦草纤维细胞浆和薄壁细胞浆的漂白特性[J]. 中国造纸, 1990, 9 (5): 26- 29.
7	翟华敏, 李忠正. 麦草纤维细胞和薄壁细胞木质素特性[J]. 中国造纸, 1992, 11 (1): 19- 22.
8	张华兰, 房桂干, 沈葵忠, 等. 慈竹薄壁组织的部分去除对化机浆制浆、漂白性能的影响[J]. 生物质化学工程, 2019, 53 (4): 31- 36. doi: 10.3969/j.issn.1673-5854.2019.04.005
9	叶利培, 房桂干, 沈葵忠, 等. 竹材薄壁细胞机械分离方法及研究进展[J]. 中华纸业, 2013, 34 (8): 26- 29. doi: 10.3969/j.issn.1007-9211.2013.08.008
10	朱玉慧, 闻靓, 张耀丽, 等. 杨木应拉木微区结构可视化及化学成分分析[J]. 林业工程学报, 2020, 5 (3): 54- 58.
11	孙海燕, 苏明垒, 王玉荣. 木材细胞壁力学性能与细胞壁组分和构造的相关性研究[J]. 林产工业, 2018, 45 (10): 22- 27.
12	LIU W, WU R, HU Y, et al. Improving enzymatic hydrolysis of mechanically refined poplar branches with assistance of hydrothermal and Fenton pretreatment[J/OL]. Bioresource Technology, 2020, 316: 123920[2022-02-20]. https://doi.org/10.1016/j.biortech.2020.123920.
13	MA C Y, XU L H, ZHANG C, et al. A synergistic hydrothermal-deep eutectic solvent(DES) pretreatment for rapid fractionation and targeted valorization of hemicelluloses and cellulose from poplar wood[J/OL]. Bioresource Technology, 2021, 341: 125828[2022-02-20]. https://doi.org/10.1016/j.biortech.2021.125828.
14	SHEN B, HOU S, JIA Y, et al. Synergistic effects of hydrothermal and deep eutectic solvent pretreatment on co-production of xylo-oligosaccharides and enzymatic hydrolysis of poplar[J/OL]. Bioresource Technology, 2021, 341: 125787[2022-02-20]. https://doi.org/10.1016/j.biortech.2021.125787.
15	储秋露, 陈雪艳, 宋凯, 等. 两步法预处理对杨木酶水解及木质素吸附性能的影响[J]. 林产化学与工业, 2019, 39 (6): 68- 74.
16	方鑫, 文沛瑶, 徐勇, 等. 亚氯酸钠和氢氧化钠两步预处理杨木制备低聚木糖和单糖[J]. 林产化学与工业, 2021, 41 (5): 25- 30.
17	文沛瑶, 游佳欣, 徐勇, 等. 杨木联产低聚木糖和枯草芽孢杆菌的研究[J]. 林产化学与工业, 2021, 41 (5): 1- 7.
18	崔晓芳. 生物化学[M]. 云南: 云南科技出版社, 2013: 44.
19	SLUITER J B , RUIZ R O , SCARLATA C J , et al. Compositional analysis of lignocellulosic feedstocks. 1.Review and description of methods[J]. Journal of Agricultural and Food Chemistry, 2010, 58 (16): 9043- 9053.
20	LI J, ZHANG H, LU M, et al. Comparison and intrinsic correlation analysis based on composition, microstructure and enzymatic hydrolysis of corn stover after different types of pretreatments[J/OL]. Bioresource Technology, 2019, 293: 122016[2022-02-20]. https://doi.org/10.1016/j.biortech.2019.122016.
21	AYENI A O , AGBOOLA O , DARAMOLA M O , et al. Kinetic study of activation and deactivation of adsorbed cellulase during enzymatic conversion of alkaline peroxide oxidation-pretreated corn cob to sugar[J]. Korean Journal of Chemical Engineering, 2021, 38 (1): 81- 89.
22	MARTTIN P G , ANNE S S T , YU-SHEN C , et al. Differential effects of inorganic salts on cellulase kinetics in enzymatic saccharification of cellulose and lignocellulosic biomass[J]. Bioprocess and Biosystems Engineering, 2021, 44 (11): 2331- 2344.
23	SAINI R , OSORIO-GONZALEZ C S , HEGDE K , et al. Lignocellulosic biomass-based biorefinery: An insight into commercialization and economic standout[J]. Current Sustainable/Renewable Energy Reports, 2020, 7 (4): 122- 136.
24	MODENBACH A A , NOKES S E . Enzymatic hydrolysis of biomass at high-solids loadings: A review[J]. Biomass and Bioenergy, 2013, 56, 526- 544.
25	LIU Z H , CHEN H Z . Biomass-water interaction and its correlations with enzymatic hydrolysis of steam-exploded corn stover[J]. ACS Sustainable Chemistry & Engineering, 2015, 4 (3): 1274- 1285.
26	JI P , JIN J , CHEN X , et al. Characterization of water state and distribution in fibre materials by low-field nuclear magnetic resonance[J]. RSC Advances, 2016, 6 (14): 11115- 11492.
27	LIU Z H , CHEN H Z . Periodic peristalsis releasing constrained water in high solids enzymatic hydrolysis of steam exploded corn stover[J]. Bioresource Technology, 2016, 205, 142- 152.
28	LIU Z H , CHEN H Z . Mechanical property of different corn stover morphological fractions and its correlations with high solids enzymatic hydrolysis by periodic peristalsis[J]. Bioresource Technology, 2016, 214, 292- 302.
29	JONKERS N , DOMMELEN J , GEERS M . Intrinsic mechanical properties of food in relation to texture parameters[J]. Mechanics of Time-Dependent Materials, 2022, 26, 323- 346.
30	邓丛静, 马欢欢, 王哲, 等. 杏壳纤维素结构表征及热解特性分析[J]. 林业工程学报, 2018, 3 (6): 87- 92.
31	游玉明, 王昱圭, 张洁, 等. 高压均质处理对竹笋膳食纤维理化性质及结构的影响[J]. 食品与发酵工业, 2021, 47 (10): 30- 36.
32	WANG N, HUANG S, ZHANG Y, et al. Effect of supplementation by bamboo shoot insoluble dietary fiber on physicochemical and structural properties of rice starch[J/OL]. LWT-Food Science & Technology. 2020, 129: 109529[2022-02-20]. https://doi.org/10.1016/j.lwt.2020.109509.

成分 composition	样品 sample	初始酶解速率/(mg·mL^-1·h^-1) initial hydrolysis rate	K_m/mg	V_max/ (mg·mL^-1·h^-1)
纤维素 cellulose	纤维细胞fiber cells	10.0	11 600	344.8
纤维素 cellulose	薄壁细胞parenchyma cells	10.7	7 600	232.6
半纤维 hemicellulose	纤维细胞fiber cells	1.4	30 954	769.2
半纤维 hemicellulose	薄壁细胞parenchyma cells	1.8	102	26.3

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