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生物质化学工程 ›› 2015, Vol. 49 ›› Issue (5): 17-22.doi: 10.3969/j.issn.1673-5854.2015.05.004

• 研究报告 • 上一篇    下一篇

稀碱-Fenton试剂预处理对云南苦竹酶水解得率的影响

王燕云1,2, 杨静1,2   

  1. 1. 西南林业大学 云南省木材胶黏剂及胶合制品重点实验室, 云南 昆明 650224;
    2. 西南林业大学 材料工程学院, 云南 昆明 650224
  • 收稿日期:2015-05-25 出版日期:2015-09-30 发布日期:2015-10-12
  • 通讯作者: 杨 静(1976—),女,博士,副教授,硕士生导师,主要从事木质纤维素的生物降解与转化的研究;E-mail:kmjingyang@163.com 。 E-mail:kmjingyang@163.com
  • 作者简介:王燕云(1991—),女,江苏苏州人,硕士生,研究方向为生物质能源
  • 基金资助:
    国家自然科学基金资助项目(31260162)

Influence of Alkaline-Fenton Pretreatment on the Enzymatic Hydrolysis Yield of Yunnan Bamboo

WANG Yan-yun1,2, YANG Jing1,2   

  1. 1. Yunnan Provincial Key Laboratory of Wood Adhesives and Glued Products, Southwest Forestry University, Kunming 650224, China;
    2. College of Materials Science and Engineering, Southwest Forestry University, Kunming 650224, China
  • Received:2015-05-25 Online:2015-09-30 Published:2015-10-12

摘要: 利用响应面法对稀碱-Fenton反应预处理竹粉的条件进行优化,确定最佳的Fenton预处理条件为:1 g 稀碱预处理后竹粉底物加入质量分数30 %的 H2O2 溶液3.4 mL,Fe2+浓度15.8 mmol/L,反应时间12 h,获得的 72 h 酶水解得率为49.98%。与原料和经2%NaOH 预处理后的样品相比,经2%NaOH-Fenton 预处理后的样品中纤维素含量升高,半纤维素和木质素含量降低,72 h酶水解得率为48.24%,分别提高了47.79和37.44个百分点。当纤维素酶和β-葡萄糖苷酶的用量分别为32 FPIU/g和16 IU/g(以纤维素质量计)时,72 h 酶水解得率为76.64%,比单独使用纤维素酶时的酶水解得率提高了22.80%。

关键词: 响应面法, Fenton反应, 酶水解得率

Abstract: The optimum reaction conditions of alkaline-Fenton pretreatment of bamboo were obtained through the response surface methodology. The results showed that the optimum conditions of Fenton reaction were alkaline treated bamboo 1 g, 30% H2O23.4 mL, concentration of Fe2+15.8 mmol/L, and reaction time 12 h. The 72 h enzymatic hydrolysis yield was 49.98%. The sample pretreated by 2% NaOH-Fenton had higher cellulose content and lower hemicellulose and lignin content compared with the materials and sample pretreated by 2% NaOH. The 72 h enzymatic hydrolysis yield was 48.24% and increased by 47.79% and 37.44%, respectively. The 72 h enzymatic hydrolysis yield was 76.64% when the dosage of the cellulase and the β-glucosidase were 32 FPIU/g and 16 IU/g, respectively. This was 22.80% higher than that with the single use of the cellulase 32 FPIU/g.

Key words: response surface methodology, Fenton reaction, enzymatic hydrolysis yield

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