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生物质化学工程

生物质化学工程 ›› 2010, Vol. 44 ›› Issue (2): 27-30.

• 论文 • 上一篇    下一篇

海南蒲桃叶黄酮的提取及抗氧化性研究

张亮亮, 陈笳鸿, 汪咏梅, 吴冬梅, 徐曼, 吴在嵩   

  1. 中国林业科学研究院, 林产化学工业研究所;生物质化学利用国家工程实验室;国家林业局, 林产化学工程重点开放性实验室, 江苏 南京 210042
  • 收稿日期:2009-12-02 修回日期:1900-01-01 出版日期:2010-03-30 发布日期:2010-03-30
  • 通讯作者: 陈笳鸿,研究员,主要从事植物资源化学利用研究;E-mail:chen-jiahong@163.com。

Study on Extraction and Antioxidant Activities of Flavones from Syzygium cumini (Linn.) Skeels Leaves

ZHANG Liang-liang, CHEN Jia-hong, WANG Yong-mei, WU Dong-mei, XU Man, WU Zai-song   

  1. Institute of Chemical Industry of Forest Products, CAF;National Engineering Lab. for Biomass Chemical Utilization;Key and Open Lab. on Forest Chemical Engineering, SFA, Nanjing 210042, China
  • Received:2009-12-02 Revised:1900-01-01 Online:2010-03-30 Published:2010-03-30

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摘要： 采用正交试验法研究了海南蒲桃叶黄酮提取条件对黄酮得率的影响,确定了最佳提取条件为:乙醇体积分数70%,料液比1:20(g:mL),提取温度80℃提取3 h,此条件下黄酮得率为6.06%。利用体外试验检测不同浓度海南蒲桃叶黄酮对二苯基苦基苯肼DPPH·的清除作用。结果表明海南蒲桃叶黄酮是一种有效的自由基清除剂,对DPPH·有显著的清除作用。黄酮溶液质量浓度在31.25～250m g/L范围内,清除率随质量浓度增大而增大,最高可达93.04%。

关键词: 海南蒲桃叶, 黄酮, 抗氧化能力, DPPH·

Abstract: Orthogonal experiments were applied to optimize the extraction conditions for flavones from Syzygium cumini Linn. Skeels leaves. The optimum extraction conditions were: ethanol volume fraction 70%, raw material to liquid ratio 1:20(g:mL), extraction temperature 80℃, extraction time 3 h, and the yield of flavones was 6.06%. The in vitro-oxidation effect on DPPH· was investigated. The results showed that S.cumini leaves was an effective free radical scavenger, and had a significant scavenging effect. The elimination rate increase with the increase of flavones concentration within the range of 31.25-250m g/L. It can reach the highest value of 93.04%.

Key words: Syzygium cumini Linn. Skeels, flavones, antioxidant capacity, DPPH·

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