飞龙掌血提取物抑菌作用初步研究

生物质化学工程 ›› 2007, Vol. 41 ›› Issue (5): 33-35.

飞龙掌血提取物抑菌作用初步研究

丁文, 文赤夫, 陈建华, 黄开兴, 董爱文

吉首大学城乡资源与规划学院, 湖南张家界 427000

收稿日期:2006-11-17 修回日期:1900-01-01 出版日期:2007-09-30 发布日期:2007-09-30

Primary Study on Antibacterial Activity of Extracts from Toddalia asiatica (Linn.)Lam

DING Wen, WEN Chi-fu, CHEN Jian-hua, HUANG Kai-xing, DONG Ai-wen

College of Resources and Programing Sciences of Jishou University, Zhangjiajie 427000, China

Received:2006-11-17 Revised:1900-01-01 Online:2007-09-30 Published:2007-09-30

摘要/Abstract

摘要： 选择蒸馏水、无水乙醇、乙酸乙酯、石油醚4种提取剂,采用冷浸、加热回流两种方法萃取飞龙掌血,比较提取物对枯草杆菌、痢疾杆菌、啤酒酵母菌的抑菌效果。结果表明,冷浸 72 h 和加热回流 5 h 的飞龙掌血提取物对该3种菌的抑菌作用无显著差异,无水乙醇、乙酸乙酯提取物较水提物有显著差异;生药质量浓度为 0.25 g/mL 的乙醇提取物对3种菌都有极显著的抑菌效果,适宜抑菌生药质量浓度为 0.5 g/mL;pH值对该提取物的抑菌效果影响较大,3个菌种适宜抑菌的pH值不同。

关键词: 飞龙掌血, 提取物, 抑菌作用

Abstract: Extracts from Toddalia asiatica (Linn.) Lam were obtained under the room temperature and hot refluxing with water, absolute alcohol,ethyl acetate and petroleum ether. Antibacterial effects of extracts from T. asiatica on Bacillus subtilis, Shigella dysenteriae and Saccharomyces cerevisiae were compared. The results showed: There was no remarkably different antibacterial activity of extracts under the room temperature for 72 h and hot refluxing for 5 h to three bacteria (p>0.05). There was remarkably different antibacterial activity of extracts of absolute alcohol and ethyl acetate in comparison with water extract (p<0.05). There was the best remarkable antibacterial effect of alcohol extracts with 0.25 g/mL crude drug (p<0.01),and the best antibacterial concentration of crude drug was 0.5 g/mL, The pH value had strong influence on antibacterial effect of extracts from T. asiatica, and the suitable pH values for inhibition of the three bacteria were different.

Key words: Toddalia asiatica(Linn.)Lam, extracts, antibacterial activity

中图分类号:

TQ91
R284.2

丁文;文赤夫;陈建华;黄开兴;董爱文. 飞龙掌血提取物抑菌作用初步研究[J]. 生物质化学工程, 2007, 41(5): 33-35.

DING Wen;WEN Chi-fu;CHEN Jian-hua;HUANG Kai-xing;DONG Ai-wen. Primary Study on Antibacterial Activity of Extracts from Toddalia asiatica (Linn.)Lam[J]. , 2007, 41(5): 33-35.

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