酵母菌的磁性载体固定化及处理精炼大豆油废水

doi:10.3969/j.issn.1673-5854.2023.01.006

摘要/Abstract

摘要：

采用发酵性丝孢酵母菌处理精炼大豆油废水, 首先利用海藻酸钠和所制备的Fe₃O₄磁性载体分别对酵母菌进行固定化, 然后分别用酵母菌、磁性载体、海藻酸钠固定化酵母菌和磁性载体固定化酵母菌来处理精炼大豆油废水。对处理结果进行对比分析, 结果显示: 4种物质中, 磁性载体固定化酵母菌对精炼大豆油废水处理36h时效果最佳。pH值6.0比较适合磁性载体固定化酵母菌的生长, 处理36h的废水中的酵母菌的OD₆₀₀值为19.78。通过研究不同pH值和不同接种量条件对磁性载体固定化酵母菌处理精炼大豆油废水的情况, 得到处理废水的较优条件: 废水pH值6.0, 酵母菌接种量10%, 处理时间36h, 此条件下废水的化学需氧量(COD)值为2430mg/L。

关键词: 发酵性丝孢酵母, 精炼大豆油废水, 废水处理, 固定化

Abstract:

This study focused on the treatment of refined soybean oil wastewater by Trichosporon fermentans. Firstly, the yeast was immobilized with sodium alginate and the prepared magnetic carrier, respectively. Then, the yeast, magnetic carrier, sodium alginate-immobilized yeast, and magnetic carrier-immobilized yeast were used to treat refined soybean oil wastewater, respectively. The comparative analysis of the treatment results showed that the magnetic carrier immobilized yeast treatment of refined soybean oil wastewater at 36 h was the best among the four substances. The pH value of 6.0 was more suitable for the growth of immobilized yeast on magnetic carrier. The OD₆₀₀ value of yeast in wastewater treated after 36 h was 19.78. By studying the treatment of refined soybean oil wastewater by magnetic carrier specified yeast under different pH values and inoculation conditions, the optimal conditions for wastewater treatment were pH value 6.0, inoculation amount 10% and culture time 36 h. Under these conditions, chemical oxygen demand value was 2 430 mg/L.

Key words: Trichosporon fermentans, refined soybean oil wastewater, wastewater treatment, immobilization

中图分类号:

TQ35
X703

吴松. 酵母菌的磁性载体固定化及处理精炼大豆油废水[J]. 生物质化学工程, 2023, 57(1): 42-48.

Song WU. Magnetic Carrier Immobilization of Yeast and Treatment of Refined Soybean Oil Wastewater[J]. Biomass Chemical Engineering, 2023, 57(1): 42-48.

图/表 6

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参考文献 17

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样品 sample	化学需氧量/(mg·L^-1) COD	含油量/(mg·L^-1) oil content	总磷/(mg·L^-1) total phosphorus	氨氮/(mg·L^-1) ammonia nitrogen	pH值 pH value
废水waste water	18 000±3 000	8 153.4±100	98.3±20	49.9±5	10.3±0.5
模拟废水simulated wastewater	20 000±2 000	8 000±200	100±15	50±5	10.0±0.5

处理时间/h treatment time	酵母菌处理废水treated by yeast			磁性载体处理废水treated by magnetic carrier
处理时间/h treatment time	pH值pH value	OD₆₀₀	COD/(mg·L^-1)	pH值pH value	COD/(mg·L^-1)
0	6.04	2.48	11 023	6.21	12 047
12	5.76	6.08	11 970	6.13	10 985
24	6.17	9.46	12 720	6.03	12 560
36	6.73	11.24	3 630	6.20	6 530
48	6.52	16.12	4 280	6.14	10 347

处理时间/h treatment time	海藻酸钠固定化酵母菌处理废水 wasterwater treated by sodium alginate immobilized yeast			磁性载体固定化酵母菌处理废水 wastewater treated by magnetic carrier immobilized yeast
处理时间/h treatment time	pH值pH value	OD₆₀₀	COD/(mg·L^-1)	pH值pH value	OD₆₀₀	COD/(mg·L^-1)
0	6.01	2.04	11 230	6.01	1.26	11 047
12	5.36	4.08	9 870	5.76	4.12	10 870
24	6.12	5.46	12 520	6.12	3.46	11 320
36	6.73	13.24	12 030	6.73	13.56	2 430
48	6.53	18.10	4 580	6.93	17.14	4 580

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