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Biomass Chemical Engineering ›› 2022, Vol. 56 ›› Issue (1): 13-22.doi: 10.3969/j.issn.1673-5854.2022.01.003

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Effect of Aerobic Biological Pretreatment Time on Hydrolytic Acidification of Corn Straw

Xiyue LI1,2, Hongbo WANG1,*(), Yuxiao ZHAO2, Dongliang HUA2, Ning WANG1   

  1. 1. School of Municipal and Environmental Engineering, Shandong Jianzhu University, Jinan 250101, Chin
    2. Energy Research Institute, Qilu University of Technology(Shandong Academy of Sciences), Jinan 250014, China
  • Received:2021-06-30 Online:2022-01-30 Published:2022-01-18
  • Contact: Hongbo WANG E-mail:wanghongbo@sdjzu.edu.cn

Abstract:

Corn straw was used as raw material for aerobic biological pretreatment by a complex bacterial system and then inoculated with anaerobic sludge for anaerobic fermentation. The influence of pretreatment time on anaerobic fermentation was investigated, and the changes in lignocellulose structure and content, key enzyme activities, microbial diversity and anaerobic acidification fermentation yield were determined. The results showed that with the extension of pretreatment time, the structure of corn straw was gradually destroyed, the activity of lignin peroxidase, the key enzyme for lignin degradation, gradually decreased, and the activities of xylanase and cellulase gradually increased and were up to 0.879 U/mg and 0.0257 U/mg. Actinomyces, Bacillus and Aspergillus were the dominant bacterium groups in the aerobic biological pretreatment of straw. The best acid production from anaerobic fermentation of corn straw was achieved by aerobic biological pretreatment for 2 d. The yield of ethanol and volatile fatty acids was 249.3 mg/g, 46.73% higher than that of untreated. The yield of ethanol and volatile fatty acids was 138.2 mg/g after 5 d of aerobic pretreatment, which was 18.66% lower than that of the untreated corn straw. The reason for the decrease of volatile fatty acids production from anaerobic fermentation due to extended aerobic pretreatment time was the excessive degradation of hemicellulose and cellulose. The pretreatment method of anaerobic fermentation of corn straw using aerobic biological pretreatment with complex microbial system as the purpose of energy and resource utilization should strictly control the pretreatment time to avoid the decrease of product yield caused by excessive degradation of cellulose and hemicellulose.

Key words: corn straw, aerobic biological treatment, anaerobic fermentation, volatile fatty acids

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