黄芩中抗菌有效成分提取工艺研究

doi:10.3969/j.issn.1673-5854.2015.05.007

Abstract

Abstract: In order to optimize the extraction condition for effective active ingredient from Scutellaria Bacalensis Georgi, a three-factor-three-level experiments design was developed by using Box-Benhken central composite design method based on single-factor experiments with extraction temperature, extraction time, solvent-solid ratio as influence factors. The bacteriostatic ring diameter against Candida albicans was used as the responsive values. The data were fitted by using multi-linear equation and second-order polynomial equation. And the extraction conditions were optimized by response surface method. The optimum conditions were as follows: extraction temperature 61 ℃, extraction time 2 h and solvent-solid ratio 13:1. Under these conditions,the average diameter of bacteriostatic circles verified by three experiments was 12.637 mm closing to the predicted value(12.664 88 mm),when the concentration of extractions was 0.5 g/mL.And the average diameter of bacteriostatic circle of positive contrast was 11.85 mm.The results showed that the aqueous extract of Scutellaria bacalensis Georgi had significant bacteriostatic effects on Candida albicans.

Key words: Scutellaria bacalensis Georgi, antibacterial ingredient, extraction, central composite design-response surface metho-dology

CLC Number:

TQ35

WANG Xue, LIU Yan-ge, LU Jia-hui, WANG Chun-yue, JIA Bing-xuan, MENG Qing-fan, ZHANG Yang, WANG Di. Extraction of Effective Ingredients from Scutellaria Bacalensis Georgi[J]. bce, 2015, 49(5): 34-38.

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Extraction of Effective Ingredients from Scutellaria Bacalensis Georgi

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