Abstract: The optimum extraction conditions of proanthocyanidins from urophylla leaves using three extraction methods were investigated by using orthogonal experiment.The extracted results were compared.Then,vitro antioxidant and anti-tumor activities of different solvent extracts from ultrasonic extracts were studied.The optimum crafts for traditional solvent extraction method were 60% ethanol,80 ℃,100 min,ratio of material-liquid 1:14(g:mL,the same below),and the obtained yield of proanthocyanidin was 5.95%.The optimum crafts for microwave-assisted extraction were 50% ethanol,microwave power 200 W,4 min for each time,ratio of material-liquid 1:20 with the yield of proanthocyanidin 5.48%.And the optimum crafts for ultrasonic extraction method were 60% ethanol,60 ℃,ultrasonic time 25 min,ratio of material-liquid 1:14,and the yield of proanthocyanidin was 6.07%.Ultrasonic assisted extraction worked best,due to the short extract time and the high yield.The results of antioxidant test showed that the ethyl acetate extracts from ultrasonic extracts exhibited the highest antioxidant activity,as well as the maximum of DPPH radical scavenging ratio was 96.33% with the concentration of 1.2 g/L among different solvent extracts.Experimental results of antitumor activity of different solvent extracts showed that the ethyl acetate extract possessed highest antitumor activity for human hepatoma cell Bel-7404 with the concentration of 2 g/L,and the inhibition rate was 56.37%.

Key words: urophylla, proanthocyanidins, extraction technology, antioxidant and antitumor activity