马占相思树皮中原花色素提取工艺及其生物活性的研究

doi:10.3969/j.issn.1673-5854.2016.06.007

Abstract

Abstract: The optimum extraction conditions of three extraction methods of proanthocyanidins from Acacia mangium Bark were investigated by using orthogonal experiment. The extracted results were compared.Then,the vitro antioxidant and anti-tumor activities of different solvent extracts from ultrasonic extracts were studied.The optimum crafts for traditional solvent extraction method were 60% ethanol,70℃,70 min and ratio of material-liquid 1:18 (g:mL,the same below),and the obtained yield of proanthocyanidin was 7.42%.The optimum crafts for microwave-assisted extraction were 60% ethanol,8 min for microware time,ratio of material-liquid 1:18,microwave power 200 W,with the yield of proanthocyanidin 7.05%.And the optimum crafts for ultrasonic-assisted extraction method were 70% ethanol,ultrasonic time 50 min,ratio of material-liquid 1:16,extraction temperature 50℃,and the yield of proanthocyanidin was 8.57%.Result of ultrasonic-assisted extraction was the best,due to the low extract temperature and high yield.The results of antioxidant test showed that the ethyl acetate extracts from ultrasonic-assisted extracts exhibited the highest antioxidant activity,as well as the inhibitory concentration 50%(IC₅₀) of DPPH·radical scavenging was 17.94 mg/L,and it was lower than that of vitamin C(Vc).Experimental results of antitumor activity of different solvent extracts showed that the ethyl acetate extract possessed highest antitumor activity for human hepatoma cell Bel-7404 with the IC₅₀ of 4.86 mg/L,which was similar to antitumor medicine etoposide.

Key words: Acacia mangium, proanthocyanidins, extraction technology, antioxidant, antitumor

CLC Number:

TQ35

XU Rui, JIANG Ping, ZHANG Ying-yin, LU Ting-ting, LI Gen, ZHANG Meng-wei. Extraction Technology of Proanthocyanidins from Acacia mangium Bark and Its Bioactivities[J]. bce, 2016, 50(6): 43-48.

References

[1] NEWAZ M S,MILLAT-E-MUSTATA M. Growth and yield prediction models for Acacia mangium grown in the plantations of the central region of Bangladesh[J]. New Forests,2004,27(1):81-88.
[2] 陈丛瑾,胡华宇,覃宇奔. 马占相思树皮制取栲胶试验[J]. 广西科学院学报,2001,17(3):100-103.
[3] 赵绍文,粱伟克,秦武明,等. 栲胶原料新品种-马占相思树皮利用研究[J]. 经济林研究,2001,19(4):38-40.
[4] ZHANG Liang-liang,CHEN Jia-hong,WANG Yong-mei,et al. Phenolic extracts from Acacia mangium bark and their antioxidant activities[J]. Molecules,2010,15(5):3567-3577.
[5] PIETARINEN S P,WILLFOR S M,SJOHOLM R E,et al. Extractives in Acacia mangium and Acacia crassicarpa stemwood and knots[C]//58th Appita Annual Conference and Exhibition Incorporating the Pan Pacific Conference:Canberra,Australia 19-21 April 2004 Proceedings,2004:557.
[6] SEIGLER D S. Phytochemistry of Acacia-sensu lato[J]. Biochemical Systematics and Ecology,2003,31(8):845-873.
[7] HOONG Y B,PIZZI A,TAHIR P M,et al. Characterization of Acacia mangium polyflavonoid tannins by MALDI-TOF mass spectrometry and CP-MAS ¹³C NMR[J]. European Polymer Journal,2010,46(6):1268-1277.
[8] HOONG Y B,PARIDAH M T,LOH Y F,et al. A new source of natural adhesive:Acacia mangium bark extracts co-polymerized with phenol-formaldehyde (PF) for bonding Mempisang (Annonaceae spp.) veneers[J]. International Journal of Adhesion and Adhesives,2011,31(3):164-167.
[9] 张慧文,张玉,马超美. 原花青素的研究进展[J]. 食品科学,2015,36(5):296-304.
[10] KRUGER M J,DAVIES N,MYBURGH K H,et al. Proanthocyanidins,anthocyanins and cardiovascular diseases[J]. Food Reaearch International,2014,59:41-52.
[11] CHEN B H,HUNG M H,CHEN J Y F,et al. Anti-allergic activity of grape seed extract (GSE) on RBL-2H3 mast cells[J]. Food Chemistry,2012,132(2):968-974.
[12] MAKINO R,OHARA S,HASHIDA K. Efficient extraction of polyphenolics from the bark of tropical tree species[J]. Journal of Tropical Forest Science,2009,21(1) 45-49.
[13] YANG Lei,SUN Xiao-wei,YANG Feng-jian,et al. Application of ionic liquids in the microwave-assisted extraction of proanthocyanidins from Larix gmelini Bark[J]. International Journal of Molecular Science,2012,13(4):5163-5178.
[14] SUN Xiao-wei,JIN Zhi-min,YANG Lei,et al. Ultrasonic-assisted extraction of procyanidins using ionic liquid solution from Larix gmelinii bark[J]. Journal of Chemistry,2013:1-9.
[15] WALLACE T,GIUSTI M. Evaluation of parameters that affect the 4-dimethylaminocinnam-aldehyde assay for flavanols and poanthocyanidins[J]. Journal of Food Science,2010,75(7):C619-C625.
[16] 姜萍,刘鑫,萧伟,等. 巨尾桉叶中原花色素提取工艺比较及不同溶剂萃取物生物活性的研究[J]. 生物质化学工程,2015,49(4):19-24.
[17] 韦献雅,殷丽琴,钟成,等. DPPH法评价抗氧化活性研究进展[J]. 食品科学,2014,35(9):317-322.
[18] 李上标,裴淑艳,蒋超,等. MTT比色法研究应用进展[J]. 西北民族大学学报:自然科学版,2013,34(3):68-73.

Extraction Technology of Proanthocyanidins from Acacia mangium Bark and Its Bioactivities

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 14

Recommended Articles

Metrics

Comments

[1]	Yonghui WU,Fashe LI,Jiaxu SHEN,Shuang WANG,Wei DU,Xuehua WU. Preparation and Kinetic Analysis of Novel Antioxidants of Biodiesel [J]. Biomass Chemical Engineering, 2020, 54(1): 31-36.
[2]	Changyi HE,Xiaohan GAO,Zewen LI,Lingling LIU,Tongtong SUN,Xiaoqiang CHEN. Compositions of the Essential Oil of Helianthus annuus Head and Its Antioxidant Activities [J]. Biomass Chemical Engineering, 2020, 54(1): 43-48.
[3]	Yishui ZHANG,Fashe LI,Shuang WANG. Comparative Analysis of Antioxidant Properties of Gallate Esters for Biodiesel Oxidation Stability [J]. Biomass Chemical Engineering, 2019, 53(5): 44-48.
[4]	Xinyan GAO,Haiying WANG,Zhiming LIU. In Vitro Antioxidant and Antibacterial Activity of Ethanol Extract from Mulberry Leaves [J]. Biomass Chemical Engineering, 2019, 53(2): 35-40.
[5]	LI Yan-fang, GAO Cai-xia, GAO Yang, HAN Xiang-en. Ultrasonic-assisted Extraction and Antioxidant Activity of Polyphenols from Koelreuteria paniculata Laxm. Leaves [J]. bce, 2016, 50(3): 27-34.
[6]	DUAN Xiao-ling, WANG Hai-ying, LIU Zhi-ming, FENG Chen, YU Sen-hai. Evaluation of Antioxidant Activities of Wood Vinegar in vitro [J]. bce, 2015, 49(6): 22-26.
[7]	JIANG Ping, LIU Xin, XIAO Wei, DING Gang, ZHAO Lin-guo. Extraction Technology of Proanthocyanidins from Urophylla Leaves and Biological Activities of Different Solvent Extracts [J]. bce, 2015, 49(4): 19-24.
[8]	JIANG Qian, ZHANG Jia-yan, QIN Yong-jian, LEI Fu-hou. Study on Fragmentation of Polymeric Proanthocyanidins from Cinnamon [J]. bce, 2015, 49(3): 34-38.
[9]	JIANG Qian, ZHANG Jia-yan, LEI Fu-hou, LIU Zu-guang. Isolation of Cinnamon Proanthocyanidins by AB-8 Macroporous Adsorption Resin [J]. bce, 2015, 49(1): 26-32.
[10]	ZHENG Yun-wu;HUANG Yuan-bo;ZHENG Zhi-feng;JIANG Li. Optimization of Extraction Technology for Rubber Seed Oil by Orthogonal Design [J]. , 2012, 46(2): 21-24.
[11]	ZHANG Liang-liang;WANG Yong-mei;XU Man;WU Dong-mei;CHEN Jia-hong. Research Advances on Analysis of Condensed Tannins from Plant Extracts [J]. , 2012, 46(2): 30-38.
[12]	BI Liang-wu;LI Da-wei;ZHAO Zhen-dong;LI Dong-mei. Review on Development and Comprehensive Utilization of Rosemary Resources [J]. , 2011, 45(3): 53-56.
[13]	LI Xue-mei;LIU Shou-qing;AO Xin-yu;LIU Xiang-yi;FU Hui. Research Progress on Antioxidation of Biodiesel [J]. , 2010, 44(4): 56-59.
[14]	ZHANG Liang-liang;CHEN Jia-hong;WANG Yong-mei;WU Dong-mei;XU Man;WU Zai-song. Study on Extraction and Antioxidant Activities of Flavones from Syzygium cumini (Linn.) Skeels Leaves [J]. , 2010, 44(2): 27-30.