化学/生物型复合增脂剂对云南松增脂效果及生理指标的影响

doi:10.3969/j.issn.1673-5854.2021.02.005

生物质化学工程 ›› 2021, Vol. 55 ›› Issue (2): 31-37.doi: 10.3969/j.issn.1673-5854.2021.02.005

化学/生物型复合增脂剂对云南松增脂效果及生理指标的影响

王瑞苓¹^,²(), 刘祥义¹^,³, 刘建祥¹^,⁴, 吴春华¹^,³^,*()

1. 西南地区林业生物质资源高效利用国家林业和草原局重点实验室, 云南昆明 650224
2. 西南林业大学林学院, 云南昆明 650224
3. 西南林业大学化学工程学院, 云南昆明 650224
4. 西南林业大学生态与环境工程学院, 云南昆明 650224

收稿日期:2020-04-15 出版日期:2021-03-30 发布日期:2021-03-23
通讯作者: 吴春华 E-mail:434745585@qq.com;906072220@qq.com
作者简介:吴春华, 教授, 博士生导师, 研究领域: 萜类化学与利用; E-mail: 906072220@qq.com
王瑞苓(1978-), 女, 河北霸州人, 讲师, 博士生, 主要从事植物生理及病理方面的研究; E-mail: 434745585@qq.com
基金资助:
国家重点研发计划资助项目(2018YFD0600401)

Effect of Chemical and Biological Compound Tapping Irritant Agent on Resin-increasing and Physical Indexes of Pinus yunnanensis

Ruiling WANG¹^,²(), Xiangyi LIU¹^,³, Jianxiang LIU¹^,⁴, Chunhua WU¹^,³^,*()

1. Key Laboratory of National Forestry and Grassland Administration on Highly-Efficient Utilization of Forestry Biomass Resources in Southwest China, Kunming 650224, China
2. School of Forestry, Southwest Forestry University, Kunming 650224, China
3. School of Chemical Engineering, Southwest Forestry University, Kunming 650224, China
4. School of Ecology and Environmental Engineering, Southwest Forestry University, Kunming 650224, China

Received:2020-04-15 Online:2021-03-30 Published:2021-03-23
Contact: Chunhua WU E-mail:434745585@qq.com;906072220@qq.com

摘要/Abstract

摘要：

以硝酸稀土为主要成分的化学增脂剂与乙烯利和α-萘乙酸这2种生物增脂剂复合得到化学/生物型复合增脂剂1和增脂剂2，并施用于云南松的增脂实验，均取得了较好的增脂效果，年平均增脂率分别为31.48%和20.20%。松脂中松节油质量分数提高24.6%，松脂流动性增加，品质得到提高。对施用增脂剂的云南松进行生理指标测试，结果表明：施用增脂剂后，松针鲜质量、干质量增加率最高可达34.57%和37.89%；叶绿素、可溶性糖和可溶性蛋白的量也都增加，最大可提高59.94%、25.88%和42.85%。云南松中三大保护酶超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、过氧化物酶（POD）活性提高，最大可提高178.38%、137.60%和43.22%，三大保护酶有助于提高植物的耐寒性和环境适应能力；施加增脂剂可以降低云南松丙二醛（MDA）含量（最多可降低36.91%），保证植物细胞内环境稳定性，从而促进云南松产脂量增加。

关键词: 云南松, 增脂剂, 过氧化物酶, 超氧化物歧化酶, 生理指标

Abstract:

The chemical tapping irritant agent with rare earth as the main component and two biological tapping irritant agents including Ethephon and α-naphthylacetic acid were applied in the experiment of increasing resin of Pinus yunnanensis. The effects of increasing resin were good, and the average increasing resin rates were 31.48% and 20.20%, respectively. The mass fraction of turpentine in resin increased by 24.6%, and the quality of resin improved. The physiological indexes of Pinus yunnanensis treated with tapping irritant agent were tested. The results showed that the maximum value of fresh weight and dry weight increased by 34.57% and 37.89%, respectively. The contents of chlorophyll, soluble sugar and soluble protein also increased, the maximum improvement rates were 59.94%, 25.88% and 42.85%, respectively. The activities of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) of Pinus yunnanensis increased with the maximum increments of 178.38%, 137.60% and 43.22%. These enzymes could help to improve cold tolerance and environmental adaptability of plants, reduce the content of malondialdehyde (MDA), with the maximum decrease rate of 36.91% to ensure the stability of plant cells, and promote resin increasing.

Key words: Pinus yunnanensis, tapping irritant agent, peroxidase, superoxide dismutase, physical indexes

中图分类号:

TQ351.44

王瑞苓, 刘祥义, 刘建祥, 吴春华. 化学/生物型复合增脂剂对云南松增脂效果及生理指标的影响[J]. 生物质化学工程, 2021, 55(2): 31-37.

Ruiling WANG, Xiangyi LIU, Jianxiang LIU, Chunhua WU. Effect of Chemical and Biological Compound Tapping Irritant Agent on Resin-increasing and Physical Indexes of Pinus yunnanensis[J]. Biomass Chemical Engineering, 2021, 55(2): 31-37.

图/表 6

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图1

表2

表3

表4

图2

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月份 months	松脂产量resin production/g			增脂率resin increasing rate/%
月份 months	0^#	1^#	2^#	1^#	2^#
1	118.80	135.40	132.40	13.97	11.45
2	157.00	186.80	169.40	18.98	7.90
3	166.60	222.00	193.20	33.25	15.97
4	146.00	211.20	207.00	44.66	41.78
5	177.60	238.60	215.80	34.35	21.51
6	133.80	210.20	167.40	57.10	25.11
7	182.20	252.80	248.20	38.75	36.22
8	202.40	265.80	234.20	31.32	15.71
9	180.20	253.20	231.80	40.51	28.63
10	140.80	173.80	161.80	23.44	14.91
11	152.00	185.00	170.00	21.71	11.84
12	129.40	146.00	136.80	12.83	5.72

月份 months	松针鲜质量/g fresh leaves weight			鲜质量增加率/% increasing rate		松针干质量/g dry leaves weight			干质量增加率/% increasing rate
月份 months	0^#	1^#	2^#	1^#	2^#	0^#	1^#	2^#	1^#	2^#
2	2.2812	2.9513	2.6893	29.37	17.89	0.7864	1.0325	0.9486	31.29	20.63
3	2.3893	2.7864	2.5934	16.62	8.54	0.8163	1.0592	0.9847	29.76	20.63
4	2.7975	3.0657	2.8846	9.59	3.11	0.8436	1.0957	1.0296	29.88	22.05
6	2.8742	3.3725	3.3064	17.34	15.04	0.8932	1.1424	1.1244	27.90	25.88
7	3.1731	3.9221	3.4672	23.60	9.27	0.9454	1.3036	1.1886	37.89	25.72
8	2.9063	3.5242	3.2344	21.26	11.29	1.1413	1.3026	1.2467	14.13	9.24
9	2.7364	3.6823	3.0635	34.57	11.95	1.1086	1.2087	1.1617	9.03	4.79
10	2.6512	3.2734	3.1365	23.47	18.30	1.1077	1.2948	1.1407	16.89	2.98
11	2.4366	2.9576	2.6537	21.38	8.91	0.9948	1.2429	1.0707	24.94	7.63
12	2.3437	2.7665	2.5696	18.04	9.64	0.9227	1.0448	0.9667	13.23	4.77

月份 month	叶绿素/(mg·g^-1) chlorophyll			可溶性糖/% soluble sugar			可溶性蛋白/(mg·g^-1) soluble protein
月份 month	0^#	1^#	2^#	0^#	1^#	2^#	0^#	1^#	2^#
2	8.34	9.21	8.13	4.89	5.15	5.02	88.98	93.77	90.15
3	6.20	7.21	6.02	4.34	4.85	4.21	51.10	73.00	64.26
4	7.17	8.53	7.14	4.13	4.27	3.86	45.51	63.85	60.62
6	6.54	10.46	10.38	2.38	2.96	2.79	72.54	79.44	70.93
7	8.46	9.80	9.48	2.29	2.88	2.65	101.92	111.38	105.19
8	7.24	8.64	8.59	2.28	2.85	2.52	72.68	79.77	75.15
9	9.04	11.02	10.58	4.56	5.28	5.05	64.75	68.25	66.77
10	8.73	9.78	9.07	4.51	5.55	5.50	65.80	71.51	71.40
11	10.30	11.10	10.80	3.69	4.28	4.08	66.60	81.19	78.64
12	10.90	12.62	11.95	3.84	4.57	4.12	56.20	68.57	65.23

月份 months	SOD/(U·g^-1·min^-1)			CAT/(mg·g^-1·min^-1)			POD/(U·g^-1·min^-1)
月份 months	0^#	1^#	2^#	0^#	1^#	2^#	0^#	1^#	2^#
2	110.65	146.70	132.90	300.98	545.72	427.93	0.71	0.87	0.73
3	89.76	178.35	119.66	272.47	488.96	299.63	0.58	0.66	0.71
4	75.94	211.40	95.07	177.76	422.36	233.89	0.45	0.58	0.64
6	71.12	117.44	98.34	168.99	398.80	296.38	0.52	0.61	0.58
7	96.55	136.83	117.20	227.15	457.17	301.73	0.54	0.67	0.62
8	96.81	139.50	116.98	231.88	466.57	309.87	0.55	0.69	0.64
9	73.91	102.26	97.19	176.16	380.27	280.75	0.53	0.65	0.60
10	104.67	133.62	123.29	225.16	426.79	336.23	0.59	0.72	0.68
11	139.56	197.79	180.31	270.80	537.63	396.37	0.60	0.85	0.75
12	157.90	226.42	198.90	306.75	602.19	441.59	0.63	0.90	0.81

[1]	王霏, 郑云武, 郑志锋. 云南松热解及其热解产物的研究[J]. 生物质化学工程, 2015, 49(4): 14-18.
[2]	张加研;张晓龙;秦永剑. 云南松树皮提取物的性能研究[J]. 生物质化学工程, 2007, 41(3): 31-33.

化学/生物型复合增脂剂对云南松增脂效果及生理指标的影响

Effect of Chemical and Biological Compound Tapping Irritant Agent on Resin-increasing and Physical Indexes of Pinus yunnanensis

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