靛蓝提取物中靛蓝和靛玉红的分析方法比较

doi:10.3969/j.issn.1673-5854.2024.01.003

Abstract

Abstract:

Using indigo paste as raw material, indigo acidification substances, glucose reducing substances, and ethyl acetate extractives were prepared using three processes of hydrochloric acid acidification, glucose reduction, and ethyl acetate extraction, respectively. High performance liquid chromatography(HPLC), ultraviolet visible spectrophotometry(UV), and dual wavelength ultraviolet spectrophotometry(dW-UV) were established to analyze the contents of indigo and indirubin in samples from three processes. The optimal analysis methods for each of three samples were determined through Kolmogorov Smirnov test, comparison of Bland Altman deviation plots, and Spearman test. The results showed that the established HPLC, UV, and dW-UV methods were simple, accurate, highly sensitive, stable, and reproducible. Three methods for detecting the content of indigo extract components all followed the normal distribution, and there were significant differences between each the two measured values. The UV method was more suitable for analyzing the content of indigo in indigo acidified substances and indigo reducing substances. HPLC method was more suitable for measuring the content of indigo and indirubin in ethyl acetate extract.

Key words: indigo, indirubin, statistic analysis, HPLC method, UV spectrometry

CLC Number:

TQ35
TQ91

Chengzhang WANG, Yangyang YAN, Hao ZHOU, Danyang LIU, Shibin SHANG, Huaxing ZHANG. Comparation of Three Methods of Analytical Methodology of Indigo and Indirubin in Different Indigo Extracts[J]. Biomass Chemical Engineering, 2024, 58(1): 18-26.

Figures/Tables 6

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References 21

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样品 samples	灰分/% ash content	酸不溶灰分/% acid-insoluble ash content	差值/% difference
靛蓝粗提物 indigo crude extract	49.53	10.58	38.95
靛蓝酸化物 acidification indigo extract	13.35	5.84	7.51
靛蓝还原物 glucose reduction indigo extract	1.70	1.59	0.11
乙酸乙酯提取物 ethyl acetate extraction indigo	3.93	2.73	0.90

分析方法 analytical method	组分 component	回归方程 linear equations	相关系数(R²) correlation coefficient	线性范围/(mg·L^-1) linear range
高效液相色谱法(HPLC)high performance liquid chromatography	靛蓝indigo	y=6.78×10⁷x+134 378	0.992	5~40
高效液相色谱法(HPLC)high performance liquid chromatography	靛玉红indirubin	y=1.25×10⁷x+75 747	0.992	3~24
紫外可见分光光度法 ultraviolet visible spectrophotometry	靛蓝indigo	y=68.31x+0.121 9	0.992	5~40
紫外可见分光光度法 ultraviolet visible spectrophotometry	靛玉红indirubin	y=38.21x+0.001 3	0.999	3~24
双波长紫外分光光度法 dual wavelength ultraviolet spectrophotometry	靛蓝(610 nm)indigo	y=68.31x+0.121 9	0.992	5~40
	靛蓝(545 nm)indigo	y=24.50x-0.003 2	0.999	5~40
	靛玉红(610 nm)indirubin	y=5.90x-0.000 3	0.999	3~24
	靛玉红(545 nm)indirubin	y=38.21x+0.001 3	0.999	3~24

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Comparation of Three Methods of Analytical Methodology of Indigo and Indirubin in Different Indigo Extracts

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