植物靛蓝提取物的分析检测方法研究进展

doi:10.3969/j.issn.1673-5854.2023.03.010

Abstract

Abstract:

Taking plant indigo extract as the research object, the research progress and trend of the detection methods for the main effective components at home and abroad were reviewed. The main components and their functions of plant indigo extract such as indigo and indirubin were summarized. The analytical examination methods of indigo extract were reviewed, mainly including high performance liquid chromatography, thin layer chromatography, thin layer scanning, dual wavelength spectrophotometry, titration analysis, gas chromatography-mass spectrometry and the combination of multiple detection methods. The application of high performance liquid chromatography(selection of chromatographic column, mobile phase, detection wavelength and evaluation parameters) was emphatically analyzed. At the same time, the problems existed in the present analysis methods were analyzed, and the future development trends of the indigo extract analysis and detection were predicted.

Key words: indigo extract, indigo naturalis, determination, indigo, indirubin

CLC Number:

TQ35
TQ91

Danyang LIU, Chengzhang WANG. Research Progress on Determination Methods of Plant Indigo Extract[J]. Biomass Chemical Engineering, 2023, 57(3): 66-72.

Figures/Tables 3

Fig.1

Table 1

Fig.2

References 54

1	鞠紫昕. 植物靛蓝染料的结构与性能研究[D]. 上海: 东华大学, 2020.
2	杨明, 张定堃, 贺亚男, 等. 青黛产业高质量发展的挑战与策略[J]. 中国中药杂志, 2021, 46 (13): 3171- 3179.
3	YANG Q Y, ZHANG T, HE Y N, et al. From natural dye to herbal medicine: A systematic review of chemical constituents, pharmacological effects and clinical applications of indigo naturalis[J/OL]. Chinese Medicine, 2020, 15(1): 127[2022-01-10]. https://doi.org/10.1186/s13020-020-00406-x.
4	SUN Q, LENG J, TANG L, et al. A comprehensive review of the chemistry, pharmacokinetics, pharmacology, clinical applications, adverse events, and quality control of indigo naturalis[J/OL]. Front Pharmacol, 2021, 12: 664022[2022-01-10]. https://doi.org/10.3389/fphar.2021.664022.
5	MILIANI C , MONICO L , FANTACCI S , et al. Photochemistry of artists' dyes and pigments: Towards better understanding and prevention of colour change in works of art[J]. Angewandte Chemie International Edition, 2018, 57 (25): 7324- 7334. doi: 10.1002/anie.201802801
6	徐春明, 李婷, 王英英, 等. 食用蓝色素及其使用现状研究进展[J]. 中国食品添加剂, 2014, (1): 208- 214.
7	杨丽群. 青黛检测方法研究进展[C]//中国药学会药物分析专业委员会. 中国药学杂志岛津杯全国药物分析优秀论文评选交流会. 2013: 262-263.
8	杨明, 刘泽玉, 苏柘僮, 等. "蓝"类植物中的前体物质转化为"靛"的机制探讨[J]. 中国中药杂志, 2010, (7): 928- 931.
9	陈雪梅. 青黛炮制过程基本原理研究[D]. 成都: 成都中医药大学, 2008.
10	马乐乐, 许润春, 张定堃, 等. 古今青黛饮片形式的变化: 靛花与粗靛质量的系统对比研究[J]. 中国中药杂志, 2021, 46 (13): 3188- 3197.
11	GO I H, CHOI T H. Characteristics of acid hydrolysis indigo extracted from indigo(Polygonum tinctorium L.) leaves[J/OL]. Palpu Chongi Gisul/Journal of Korea Technical Association of the Pulp and Paper Industry, 2016, 48(3): 57[2022-01-10]. https://doi.org/10.7584/ktappi.2016.48.3.057.
12	张婷, 黄胜杰, 邓璇, 等. 基于聚乙二醇表面成膜法的青黛亲水性饮片制备及系统评价[J]. 中国中药杂志, 2021, 46 (13): 3213- 3221.
13	徐先祥, 刁勇, 周心怡. 一种青黛的制备方法: CN109364126A[P]. 2019-02-22.
14	黄慧, 倪峰, 郭丹, 等. 基于UPLC指纹图谱的青黛提取工艺评价研究[J]. 中国现代应用药学, 2017, 34 (4): 533- 537.
15	石岩, 魏锋, 马双成. 关于青黛来源、制法及质量问题的探讨[J]. 中国中药杂志, 2019, (3): 608- 613.
16	杨阳, 浦如月, 冯格, 等. 高效液相色谱法同时测定马蓝根、茎、叶中4种成分的含量及其3种不同干燥方法的评价[J]. 中国医院药学杂志, 2018, 38 (2): 120- 125.
17	王婷婷, 李玲, 陈乃江. 高效液相色谱法测定双料喉风散中靛蓝和靛玉红含量[J]. 中国药业, 2019, 28 (23): 24- 26. doi: 10.3969/j.issn.1006-4931.2019.23.007
18	唐吉. 高效液相色谱法测定复肝宁胶囊中靛玉红含量[J]. 现代医药卫生, 2012, 28 (4): 501- 502.
19	林胤谷. 青黛的经油萃取的产物及其制备方法与用途: CN105708874A[P]. 2016-06-29.
20	高凤洋, 张大方, 李超英. 中药青黛炮制及药理作用的研究进展[J]. 长春中医药大学学报, 2020, 36 (1): 180- 183.180-183, 188
21	侯宪邦. 板蓝根潜在药效成分的发现及其作用机制的研究[D]. 南京: 南京中医药大学, 2017.
22	黄远, 李菁, 徐科一, 等. 板蓝根抗流感病毒有效成分研究进展[J]. 中国现代应用药学, 2019, 36 (20): 2618- 2623.
23	闫峻, 顾娟, 冯硕, 等. 大青叶化学成分及抗氧化活性研究[J]. 质谱学报, 2019, 40 (4): 381- 390.
24	徐志琴, 蔡铱妮, 陈奕弟, 等. 马蓝叶化学成分研究[J]. 中药材, 2021, (8): 1876- 1880.
25	GABORIAUD-KOLAR N , VOUGOGIANNOPOULOU K , SKALTSOUNIS A L . Indirubin derivatives: A patent review(2010-present)[J]. Expert Opinion on Therapeutic Patents, 2015, 25 (5): 583- 593.
26	林琳, 刁勇, 周心怡, 等. 靛蓝的生物活性研究进展[J]. 染料与染色, 2019, 56 (4): 16- 18.
27	STASIAK N , KUKUA-KOCH W , GOWNIAK K . Modern industrial and pharmacological applications of indigo dye and its derivatives: A review[J]. Acta Poloniae Pharmaceutica, 2014, 71 (2): 215- 221.
28	高凤洋, 张大方, 李超英. 中药青黛炮制及药理作用的研究进展[J]. 长春中医药大学学报, 2020, 36 (1): 180- 183.180-183, 188
29	刘远, 欧阳富, 于海洋, 等. 马蓝叶化学成分研究[J]. 中国药物化学杂志, 2009, 19 (4): 273- 275.273-275, 283
30	国家药典委员会. 中华人民共和国药典: 一部[M]. 北京: 中国医药科技出版社, 2020.
31	侯惠婵, 梁少珍. HPLC法测定马蓝根, 茎, 叶中靛玉红, 靛蓝的含量[J]. 中药材, 2006, 29 (7): 681- 682.
32	张先达, 陈奕龙, 张丹雁. HPLC法同时测定南大青叶中5种有效成分的含量[J]. 广东药学院学报, 2016, 32 (4): 449- 453.
33	李大伟, 毕良武, 赵振东, 等. 迷迭香提取物检测方法研究进展[J]. 林产化学与工业, 2011, 31 (2): 119- 126.
34	裴玉, 黄芝娟, 霍志鹏, 等. 多波长HPLC法同时测定青黛药材中靛蓝和靛玉红的含量[J]. 天津药学, 2017, 29 (2): 14- 18.
35	孙媛, 田秀秀, 丁江生. 南板蓝根不同提取物中靛蓝, 靛玉红和色胺酮含量及其抑菌活性的研究[J]. 中医药通报, 2021, 20 (3): 63- 67.
36	罗巍伟, 贺英菊, 王凌, 等. HPLC测定板蓝根提取物中靛蓝和靛玉红的含量[J]. 华西药学杂志, 2004, (6): 455- 456.
37	潘淑琴. 菘蓝中靛蓝与靛玉红代谢积累规律的研究[D]. 西安: 陕西师范大学, 2015.
38	MOHN T , POTTERAT O , HAMBURGER M . Quantification of active principles and pigments in leaf extracts of Isatis tinctoria by HPLC/UV/MS[J]. Planta Medica, 2007, 73 (2): 151- 156.
39	蒋一苇, 郭娜, 刘志国, 等. 蓝靛果籽油提取工艺优化及其脂肪酸组成分析[J]. 林产化学与工业, 2020, 40 (6): 30- 36.
40	王巍嵩, 朱贲贲, 徐智宇. 高效液相色谱-质谱法测定大青叶中靛蓝和靛玉红的含量[J]. 内蒙古医科大学学报, 2020, 42 (1): 59- 60.59-60, 63
41	周心怡. 建青黛加工工艺及质量标准研究[D]. 厦门: 华侨大学, 2019.
42	张丁华, 王艳丰. 中药指纹图谱技术及应用研究进展[J]. 中兽医医药杂志, 2010, 29 (1): 26- 28.
43	刘东方, 赵丽娜, 李银峰, 等. 中药指纹图谱技术的研究进展及应用[J]. 中草药, 2016, 47 (22): 4085- 4094.
44	李媛, 张苗苗, 张鑫, 等. 多成分含量测定结合高效液相色谱指纹图谱评价不同产地大青叶药材质量[J]. 中国药业, 2019, 28 (17): 15- 18.
45	叶桦珍, 黄慧, 黄建凡, 等. 建青黛UPLC指纹图谱的建立及鉴别研究[J]. 福建分析测试, 2016, 25 (4): 6- 13.
46	董娟娥, 龚明贵, 梁宗锁. 板蓝根, 大青叶中靛蓝和靛玉红的测定方法比较[J]. 西北农林科技大学学报, 2007, 35 (2): 215- 219.
47	宗玉英, 党合群. 薄层扫描法测定复方板蓝根胶囊中靛玉红含量[J]. 青海医药杂志, 2000, 30 (3): 52- 53.
48	刘依, 韩鲁佳, 阎巧娟, 等. 板蓝根中靛蓝和靛玉红的提取及其质量分数的测定[J]. 中国农业大学学报, 2003, (6): 5- 8.
49	刘泽玉. 青黛炮制过程中原理与工艺的优化研究[D]. 成都: 成都中医药大学, 2011.
50	程力惠, 李晓蒙. 青黛的质量鉴别[J]. 中医药导报, 2006, 12 (12): 77.77, 87
51	DEGANI L , RIEDO C , CHIANTORE O . Identification of natural indigo in historical textiles by GC-MS[J]. Analytical & Bioanalytical Chemistry, 2015, 407 (6): 1695- 1704.
52	CASAS-CATALÁN M J , DOMÉNECH-CARBÓ M T . Identification of natural dyes used in works of art by pyrolysis-gas chromatography/mass spectrometry combined with in situ trimethylsilylation[J]. Analytical and bioanalytical chemistry, 2005, 382 (2): 259- 268. doi: 10.1007/s00216-005-3064-0
53	LÜ H T , LIU J , DENG R , et al. Preparative isolation and purification of indigo and indirubin from folium isatidis by high-speed counter-current chromatography[J]. Phytochemical Analysis, 2012, 23 (6): 637- 641.
54	刘娟汝, 刘晓梅, 刘雨诗, 等. 基于色度分析原理的青黛有效成分含量与其色度值的相关性分析[J]. 中国实验方剂学杂志, 2019, 25 (23): 165- 170.

有效成分 active constituent	原料 substrate	流动相(体积比) mobile phase(volume ratio)	检测波长/nm detection wavelength	参考文献 ref.
靛蓝 indigo	大青叶 isatidis folium	甲醇/水 methanol/water(75∶25)	289	[30]
	蓼大青叶 polygoni tinctorii folium	甲醇/水 methanol/water(60∶40)	604	[30]
	青黛 indigo naturalis	甲醇/水 methanol/water(75∶25)	606	[30]
	马蓝根、茎、叶 root, stem and leaf of Baphicacanthus cusia(Nees) Bremek.	甲醇/水 methanol/water(75∶25)	289	[31]
靛玉红 indirubin	大青叶 isatidis folium	甲醇/水 methanol/water(75∶25)	289	[30]
	蓼大青叶 polygoni tinctorii folium	甲醇/水 methanol/water(60∶40)	604	[30]
	青黛 indigo naturalis	甲醇/水 methanol/water(70∶30)	292	[30]
	南大青叶 leaves of Baphicacanthus cusia(Nees) Bremek.	乙腈/水(梯度洗脱)acetonitrile/water(gradient elution)	225	[32]
	马蓝根、茎、叶 root, stem and leaf of Baphicacanthus cusia(Nees) Bremek.	甲醇/水 methanol/water(75∶25)	289	[31]
(R, S)-告依春 (R, S)-goitrin	板蓝根 isatidis radix	甲醇/0.02%磷酸溶液 methanol/0.02% phosphoric acid solution(7∶93)	245	[30]
色胺酮 tryptanthrin	南大青叶 leaves of Baphicacanthus cusia(Nees) Bremek.	乙腈/水(梯度洗脱) acetonitrile/water(gradient elution)	225	[32]

[1]	XU Man, WANG Yongmei, ZHANG Liangliang, HU Xinyu. Quantitative Determination of 3,4,5-Trimethoxybenzoic Acid [J]. bce, 2018, 52(1): 23-28.
[2]	CHU Hong-ying;CUI Hong;YUAN Qi. Method for the Determination of Vitamin C and Its Content Changes in Sonchus oleraceus L. [J]. , 2012, 46(3): 9-12.
[3]	XU Man;CHEN Jia-hong;WANG Yong-mei;WU Dong-mei;WU Zai-song;ZHANG Liang-liang;. Quantitative Determination of Dodecyl Gallate [J]. , 2011, 45(1): 25-28.
[4]	WU Dong-mei;CHEN Jia-hong;WANG Yong-mei;ZHANG Liang-liang;XU Man. Quantitative Determination of Methyl Gallate by HPLC Method [J]. , 2010, 44(6): 6-9.
[5]	SUN Hong;ZHANG Ze. Progress on the Determination of Natural Polyphenols by Spectrophotometric Analysis [J]. , 2008, 42(3): 55-58.
[6]	ZHAO Zhen-dong;LI Dong-mei;BI Liang-wu;WANG Jing;GU Yan;LIU Xian-zhang. Comparison of Determination Methods of Camphor Content in Synthetic Camphor [J]. , 2008, 42(1): 1-5.
[7]	XU Xue-sheng;CHEN Zheng-hua. Determination of Trace Arsenic in Activated Carbon [J]. , 2007, 41(4): 43-45.
[8]	CHEN Jia-hong;WU Dong-mei;WANG Yong-mei;WU Zai-song. Study on Quantitative Determination of Ellagic Acid by UV-spectrophotometry [J]. , 2007, 41(3): 18-20.
[9]	DENG Su-lan;YU Ji-hong;GUAN Lin. The Determination of Flavonoids in Different Parts of Eucommia Ulmoides Oliver [J]. , 2007, 41(3): 37-38.
[10]	WU Dong-mei;CHEN Jia-hong;WANG Yong-mei;WU Zai-song. Study on Quantitative Determination of Pyrogallic Acid [J]. , 2007, 41(2): 41-43.
[11]	SHI Xiang-li;LI Meng-zhu. Rapid Determination of Iron in Rosin [J]. , 2006, 40(2): 25-26.

Research Progress on Determination Methods of Plant Indigo Extract

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 3

References 54

Related Articles 11

Recommended Articles

Metrics

Comments