杨木MWL和LCC制备多孔生物载体及其在人体肝细胞培养中的应用

doi:10.3969/j.issn.1673-5854.2016.05.002

bce ›› 2016, Vol. 50 ›› Issue (5): 7-11.doi: 10.3969/j.issn.1673-5854.2016.05.002

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Preparation of Porous Biological Carrier with Aspen MWL and LCC and Application in Culture of Human Hepatocytes

WU Chen-xi¹, YE Zhe-zi¹, LE Xi¹, WANG Peng^1,2, XIE Yi-min^1,2

1. School of pulp & Paper Engineering, Hubei University of Technology, Wuhan 430068, China;
2. Hubei Provincial Key Laboratory of Green Materials for Light Industry, Hubei University of Technology, Wuhan 430068, China

Received:2016-02-23 Online:2016-09-30 Published:2016-12-03

Abstract

Abstract: In order to compare the biocompatibility of milled wood lignin (MWL) and lignin-carbohydrate complexes(LCC),MWL and LCC were prepared with aspen wood in the present research.Hydrogels were prepared with these materials.Then,porous biological carriers were obtained by freeze-drying.Fourier transform infrared spectrometer(FT-IR) and scanning eletron microscope(SEM) were applied to elucidate the composition and morphology of the porous biological carriers.Inverted microscope,albumin and glucose kits were used to monitor the morphology and metabolic activity of the cultured hepatocytes.The results indicated that LCC based carriers contained more carbohydrates and less lignin.Therefore,its amphipathicity is better than the MWL based carriers.The porosity of the MWL and LCC based carriers was about 60% with average pore size of 30 μm.The results of analysis of cell growth,albumin and glucose metabolism showed that porous biological carriers prepared with aspen LCC could promote the growth of human hepatocytes during culture signficantly. Its biocompatibility is better than that of MWL.

Key words: milled wood lignin, lignin-carbohydrate complexes, biological carrier, hepatocytes, biocompatibility

CLC Number:

TQ35

WU Chen-xi, YE Zhe-zi, LE Xi, WANG Peng, XIE Yi-min. Preparation of Porous Biological Carrier with Aspen MWL and LCC and Application in Culture of Human Hepatocytes[J]. bce, 2016, 50(5): 7-11.

References

[1] 邬义明. 植物纤维化学[M]. 北京:中国轻工业出版社,1999.
[2] O'SULLIVAN A C. Cellulose:The structure slowly unravels[J]. Cellulose,1997,4(3):173-207.
[3] ERAKOVIC S,VELJOVIC D,DIOUF P N,et al. Electrophoretic deposition of biocomposite lignin/hydroxyapatite coatings on titanium[J]. International Journal of Chemical Reactor Engineering,2009,7(1):91-97.
[4] MANSUR H S,MANSUR A A P,BICLLHO S M C M. Lignin-hydroxyapatite/tricalcium phosphate biocomposites:SEM/EDX and FTIR characterization[J]. Key Engineering Materials,2005,284/285/286:745-748.
[5] MARTINEZ M M,PACHECO B A,VARGAS V M. Histological evaluation of the biocompatibility and bioconduction of a hydroxyapatite-lignin compound inserted in rabbits shinbones[J]. Revista Mvz Córdoba,2009,14(1):1624-1632.
[6] 裴继诚. 植物纤维化学[M]. 北京:中国轻工业出版社,2012.
[7] SINGH R,SINGH S,TRIMUKHE K D,et al. Lignin-carbohydrate complexes from sugarcane bagasse:Preparation,purification,and characterization[J]. Carbohydrate Polymers,2005,62(1):57-66.
[8] 刘利. 高分子肝支架材料半乳糖化壳聚糖的制备和研究[D]. 武汉:武汉理工大学硕士学位论文,2007.
[9] TSUJI R,KOIZUMI H,AOKI D,et al. LREL,a cellulase-treated lignin-carbohydrate derived from plants,activates myeloid dendritic cells via TLR4[J]. The Journal of Biological Chemistry,2014,290(7).
[10] 李金玲,王鹏,杨静,等. 银杏LCC制备多孔生物载体及在人体肝细胞培养中的应用[J]. 林产化学与工业,2014,34(6):81-87.
[11] 李金玲. LCC支架材料的制备及其在人体正常肝细胞L-02培养中的应用[D]. 武汉:湖北工业大学硕士学位论文,2014.
[12] 何君仁,杨自权,李刚,等. 脱细胞软骨生物支架材料与大鼠骨髓间充质干细胞共培养及体内埋植后的降解[J]. 中国组织工程研究与临床康复,2008,12(27):5230-5234.
[13] 张瑞. 壳聚糖球形多孔微载体制备及其肝细胞培养的初步研究[D]. 上海:上海交通大学博士学位论文,2009.
[14] SHARON G,BURDICK J A,FERREIRA L S,et al. Hyaluronic acid hydrogel for controlled self-renewal and differentiation of human embryonic stem cells[J]. Proceedings of the National Academy of Sciences,2007,104(27):11298-11303.
[15] BJÖRKMAN A. Studies on finely divided wood. Part 1:Extraction of lignin with neutral solvents[J]. Svensk papperstidning,1956,59(13):477-485.
[16] 刘玉琴. 细胞培养实验手册[M]. 北京:人民军医出版社,2009.

Preparation of Porous Biological Carrier with Aspen MWL and LCC and Application in Culture of Human Hepatocytes

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