发酵性丝孢酵母处理精炼大豆油废水的工艺优化及动力学分析

doi:10.3969/j.issn.1673-5854.2022.05.008

Abstract

Abstract:

The treatment of the refined soybean oil wastewater by Trichosporon fermentans was optimized by orthogonal test in a 5 L stirred reactor. By the range analysis, the optimal conditions for biological treatment of refined soybean oil wastewater were temperature of 28 ℃, rotating speed of 300 r/min, air intake of 2 L/min, and inoculation amount of 10%. After 36 h operation under these conditions, the removals of COD and oil content of the refined soybean oil wastewater were 97.31% and 89.09%, and the biomass and lipid content reached 9.27 g/L and 51.9%, respectively. The Monod, Tessier, and Moser models were applied to study the biomass growth of Trichosporon fermentans and the pollutant degradation of the refined soybean oil wastewater, and it was found that the Tessier model was more suitable for studying the growth of microorganisms than those of the Monod and Moser models. The kinetic equations for the biomass growth of Trichosporon fermentans were established, and the corresponding parameters were obtained and applied to assess the design and operation of a bioreactor for the treatment of oily wastewater.

Key words: Trichosporon fermentans, refined soybean oil wastewater, biological water treatment, orthogonal test, kinetic analysis

CLC Number:

TQ35
X703

Song WU. Technical Study and Kinetic Analysis of the Treatment of Refined Soybean Oil Wastewater by Trichosporon fermentans[J]. Biomass Chemical Engineering, 2022, 56(5): 51-57.

Figures/Tables 7

Table 1

Table 2

Fig.1

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Table 4

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Fig.3

References 18

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样品 sample	COD/ (mg·L^-1)	含油量/(mg·L^-1) oil content	总磷/(mg·L^-1) total phosphorus	氨氮/(mg·L^-1) ammonia-nitrogen	pH值 pH value
精炼大豆油废水refined soybean oil wastewater	18 000±3 000	8 153.4±100	98.3±20	49.9±5	10.3±0.5
模拟废水 simulated wastewater	20 000±2 000	8 000±200	100±15	50±5	10.0±0.5

试验号 No.	A 温度/℃ temperature	B 转速/(r·min^-1) rotating speed	C 进气量/(L·min^-1) air input	D 接种量/% inoculation amount	COD 去除率/% COD removal rate
1	26	100	1	5	25.15
2	26	300	2	10	95.68
3	26	500	3	15	59.62
4	28	100	2	15	54.95
5	28	300	3	5	73.15
6	28	500	1	10	80.63
7	30	100	3	10	60.72
8	30	300	1	15	76.25
9	30	500	2	5	53.88
k₁	60.150	46.940	60.677	50.727
k₂	69.577	81.693	68.170	79.010
k₃	63.617	64.710	64.497	63.607
R	9.427	34.753	7.493	28.283

序号 No.	S₀/ (mg·L^-1)	S_e/ (mg·L^-1)	U₀/ (mg·L^-1)	U_e/ (mg·L^-1)	X₀/ (mg·L^-1)	X_e/ (mg·L^-1)	μ/ (h^-1)	q/ (h^-1)	p/ (h^-1)
1	20 140	4 950	4 973.2	1 143.3	1 825	8 610	0.043 1	0.096 5	0.024 3
2	17 920	3 640	4 095.1	1 023.8	1 825	8 310	0.042 1	0.087 3	0.019 9
3	14 790	2 670	3 157.6	779.4	1 825	7 850	0.040 5	0.081 6	0.016 0
4	10 260	1 945	2 093.5	513.4	1 825	5 060	0.028 3	0.072 9	0.013 8
5	7 200	890	1 109.8	266.5	1 825	3 740	0.019 9	0.065 7	0.008 8
6	4 420	650	586.9	132.7	1 825	2 989	0.013 7	0.044 4	0.005 3

	模型model	参数parameter	R²	F值F value	P
Monod	μ=μ_maxS/(K_{s, μ}+S)	μ_max=0.116 K_{s, μ}=31 737.480	0.969	679.001	1.289×10^-5
	q=q_maxS/(K_{s, q}+S)	q_max=0.129 K_{s, q}=7 866.402	0.965	1 508.013	2.627×10^-6
	μ=μ_maxU/(K_{u, μ}+U)	μ_max=0.067 K_{u, μ}=2 507.618	0.971	716.867	1.157×10^-5
	p=p_maxU/(K_{u, p}+U)	p_max=0.048 K_{u, p}=5 474.688	0.969	502.337	2.346×10^-5
Tessier	μ=μ_max(1-e^{-S/K_{s, μ}})	μ_max=0.070 K_{s, μ}=19 504.089	0.971	739.147	1.088×10^-5
	q=q_max(1-e^{-S/K_{s, q}})	q_max=0.098 K_{s, q}=7 140.664	0.957	1 213.106	4.055×10^-6
	μ=μ_max(1-e^{-U/K_{u, μ}})	μ_max=0.048 K_{u, μ}=2 042.450	0.973	773.142	9.952×10^-6
	p=p_max1-e^{-U/K_{u, p}}	p_max=0.031 K_{u, p}=3 857.028	0.964	428.562	3.217×10^-5
Moser	μ=μ_maxSⁿ/(K_{s, μ}ⁿ+Sⁿ)	μ_max=0.062 K_{s, μ}=11 085.484 n_μ=1.515	0.972	496.724	1.652×10^-4
	q=q_maxSⁿ/(K_{s, q}ⁿ+Sⁿ)	q_max=0.125 K_{s, q}=7 362.663 n_q=1.046	0.954	755.638	8.818×10^-5
	μ=μ_maxUⁿ/(K_{u, μ}ⁿ+Uⁿ)	μ_max=0.067 K_{u, μ}=2 474.119 n_μ=1.006	0.961	358.467	2.690×10^-4
	p=p_maxUⁿ/(K_{u, p}ⁿ+Uⁿ)	p_max=23.351 K_{u, p}=1.248×10⁸ n_p=0.681	0.977	453.538	1.893×10^-4

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Technical Study and Kinetic Analysis of the Treatment of Refined Soybean Oil Wastewater by Trichosporon fermentans

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