Abstract: Methods of enrichment of azadirachtin by solvent and multi-solvent partition were studied, The optimized condition of multi-solvent partition of azadirachtin obtained by orthogonal tests was: solvent: dichloromethane: ethyl acetate(1:5,V:V), separatory liquid: acetone:water(1:1), solvent:separatory liquid(10:3). Multi-solvent partition was found to be able to enhance the content of azadirachtin in ethanol extract from 3.68%-17.76%. It is a simple and efficient method to enrich azadirachtin in its extracts.

Key words: multi-solvent partition, azadirachtin, enrichment