Abstract: The glabridin liposome were prepared by the complex coacervation method with glabridin as raw material.Taking the encapsulation rate and particle size distribution as the indexes,the preparation technology of glabridin liposome was optimized by orthogonal design.The particle size distribution,release characteristics in vitro and stability of liposome were studied.The results showed that the optimal preparation conditions were the mass ratio of lecithin and cholesterol 4:1,ultrasonic emulsific time 40 min, and mass concentration of glabridin propylene glycol solution 8 g/L.Under these conditions,the obtained glabridin liposome had uniform particle size distribution and the percentage of particle size 0.1-1.2 μm was 84.67%.The comprehensive score of the encapsulation rate and particle size was over 78.The release charactistic of liposome was better than that of glabridin powder in the first 120 min and the mass fraction of glabridin at 3 months was 38.5% with the retention rate of 96.25%.Complex coacervation used to prepare glabridin liposome was simple and reliable,and the product was stable.As a new natural additive for whitening cosmetics,glabridin liposome had certain marketing significance.

Key words: glabridin, liposome, complex coacervation method, preparation technology, in vitro release