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›› 2013, Vol. 47 ›› Issue (2): 9-14.doi: 10.3969/j.issn.1673-5854.2013.02.003

• 研究报告 • Previous Articles     Next Articles

Process of Neutral Cellulase-based Saccharification on the Neutral Steam Exploded Corn Stover

ZHONG Jian1,2, YUE Yang1,2, SHI Jia-ji1, SUN Yan2, CHAO Ya-peng1, QIAN Shi-jun1   

  1. 1. National Engineering Lab for Industrial Enzymes, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China;2. School of Biological Science and Medical Engineering, BUAA, Beijing 100191, China
  • Received:2012-08-20 Revised:1900-01-01 Online:2013-03-30 Published:2013-03-30

Abstract: A neutral cellulase was introduced to optimize the saccharification process of neutral steam exploded corn stover. It was found that the neutral cellulase was affected equally in the non-buffer and buffer system under the natural condition. Further investigation showed that xylase, β-glucosidase, and Tritonx-100 have positive effects on the saccharification in the system. In summary, an optimized enzymatic hydrolyzing condition for one gram neutral steam exploded corn stover (100 g/L) is obtained as follows: 10 FPU neutral cellulase complex, 75 IU β-glucosidase, 3 000 IU xylase and Triton-100 with 0.5 percent in volume. After 120 h incubation at 50 ℃ with 150 r/min,total biomass conversion is around 48.4%. The total biomass conversion and cellulose conversion rates are 56.0% and 64.9% when using two-step addition of the enzymes.

Key words: neutral cellulase, neutral steam exploded corn stovers, synergic effects, saccharification

CLC Number: