Abstract: Orange peel was extracted by saturated calcium hydroxide solution through microwave-assisted extraction or ultrasonic extraction. The yield and extraction rate of hesperidin were used to evaluate the effect of tests. The optimum extraction conditions of microwave-assisted and ultrasound method were obtained and compared by single factor test and orthogonal test. The results showed that the ultrasonic extraction method is superior to microwave-assisted extraction method, and the optimum extraction conditions of ultrasonic were as follows: extraction temperature 20℃, extraction time 70 min, liquid-solid ratio 30:1. Compared with conventional extraction, the yield of hesperidin reached to 5.28%, increased four times, and the extraction rate of hesperidin reached to 78.43%,enchanced 58.21 percentage point, the results of larger experiment is relatively stable by ultrasonic extraction.

Key words: hesperidin, microwave-assisted extraction, ultrasonic extraction, comparation