Abstract: Research progress of immobilized cellulases with soluble, insoluble and souble-insoluble carriers was mainly introduced. Cellulase immobilized on soluble carriers can hydrolyze insoluble cellulose effectively, but cellulase can't be recycled expediently. Cellulase immobilized on insoluble carriers could be recycled expediently, but most of them can't hydrolyze insoluble cellulose because of poor contact. Even immobilized on magnetic nanoparticles and membranes, it could only hydrolyze soluble CMC and not hydrolyze insoluble cellulose efficiently. Immobilized cellulase on soluble-insoluble(S-IS) carriers can hydrolyze insoluble cellulose, and be recycled expediently, but cellulase is difficult to be combined on S-IS firmly and the activity and amount loss of the immobilized cellulase is significant during precipitation-dissolution process. It is expected to develop new S-IS and immobilization method.

Key words: immobilized cellulase, soluble carrier, insoluble carrier, soluble-insoluble carrier