油茶叶多糖的闪式提取工艺优化及其抗氧化活性

doi:10.3969/j.issn.1673-5854.2021.05.007

Abstract

Abstract:

On the basis of single factor experiment, the flash extraction process of polysaccharide from the leaves of Camellia oleifera Able. was optimized by response surface methodology, and the antioxidant activity of polysaccharide was investigated. The optimum conditions of flash extraction were as follows: material-liquid ratio 1:30 (g: mL), temperature 81℃, extraction time 75 s. Under these conditions, the extraction rate of polysaccharide was 8.43%. The polysaccharide from the leaves of Camellia oleifera Able. had strong scavenging ability to DPPH·, ·ABTS⁺ and hydroxyl radicals, with the inhibitory concentration 50% (IC₅₀) values of 1.706, 0.826 and 4.811 g/L, respectively. Therefore, it had good antioxidant activity.

Key words: leaves of Camellia oleifera Able., polysaccharide, response surface methodology, antioxidant activity

CLC Number:

TQ35
O629.3

Run YE, Jing CAI, Jinzhe LI, Wei SUN, Jiapeng SHEN. Optimization of Flash Extraction Technology of Polysaccharide from Leaves of Camellia oleifera Able. and Its Antioxidant Activity[J]. Biomass Chemical Engineering, 2021, 55(5): 42-46.

Figures/Tables 5

Fig.1

Table 1

Table 2

Fig.2

Fig.3

References 15

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试验号 No.	A 料液比(g∶mL) material-liquid ratio	B 提取温度/℃ extraction temperature	C 提取时间/s extraction time	Y 多糖得率/% yield of polysaccharide
1	1∶20	90	70	7.83
2	1∶20	70	70	7.69
3	1∶30	80	70	8.15
4	1∶40	80	60	7.68
5	1∶30	80	70	8.18
6	1∶30	90	80	7.92
7	1∶30	80	70	8.16
8	1∶30	90	60	7.67
9	1∶20	80	60	7.64
10	1∶30	80	70	8.14
11	1∶40	80	80	7.95
12	1∶40	90	70	7.94
13	1∶30	80	70	8.18
14	1∶20	80	80	7.92
15	1:30	70	60	7.52
16	1∶30	70	80	7.88
17	1∶40	70	70	7.72

变异来源 origin	平方和 sum of square	自由度degrees of freedom	均方 mean square	F值 F value	P值 P value	显著性 significance
模型model	0.74	9	0.082	96.33	＜0.0001	**
A	5.513×10^-3	1	5.513×10^-3	6.48	0.0384	*
B	0.038	1	0.038	44.45	0.0003	**
C	0.17	1	0.17	197.72	＜0.0001	**
AB	1.600×10^-3	1	1.600×10^-3	1.88	0.2126
AC	2.500×10^-5	1	2.500×10^-3	0.029	0.8687
BC	3.025×10^-3	1	3.025×10^-3	3.56	0.1013
A²	0.11	1	0.11	124.34	＜0.0001	**
B²	0.18	1	0.18	215.16	＜0.0001	**
C²	0.18	1	0.18	210.03	＜0.0001	**
残余residual	5.955×10^-3	7	8.507×10^-4
失拟项lack of fit	4.675×10^-3	3	1.558×10^-3	4.87	0.0801
绝对误差pure error	1.280×10^-3	4	3.200×10^-4
总和总离差total value	0.74	16

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Optimization of Flash Extraction Technology of Polysaccharide from Leaves of Camellia oleifera Able. and Its Antioxidant Activity

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