离子色谱法测毛竹竹叶单糖和糖醛酸组成

doi:10.3969/j.issn.1673-5854.2019.06.006

Abstract

Abstract:

A simple, rapid and accurate method for the determination of monosaccharide compositions from lignocellulose byionchromatographywas established. The HPAEC-PAD method was established to simultaneously detect 7 different monosaccharides and two types of alduronic acid. Dionex ICS-5000⁺ with CarboPAC^TMPA10(4 mm×250 mm) column and pulsed amperometric detection was involved in this method. This method could be simultaneously qualitatively and quantitatively analysed by the gradient elution of NaOH and CH₃COONa.The results showed that the method has good linearity (R²≥0.999 0), high precision (RSD < 3%), adequate (98.53%-118.63% recovery) and good reproducibility (RSD < 1.16%)within the range. The limitions of quantitation and detection were 0.05-3.0 mg/L and 0.01-1 mg/L, respectively. The method was applied to determine the monosaccharide compositions from bamboo leaves hydrolysate.The results showed that bamboo leaves hydrolysate was composed of arabinose, galactose, glucose and xylose.The mole ratio of monosaccharide to n(arabinose) : n(galactose) : n(glucose) : n(xylose)=2.9 : 0.7 : 8.2 : 9.7.This method is successful and can be used for the determination of monosaccharides in lignocellulose.

Key words: HPAEC-PAD method, lignocelluloses, monosaccharide, uronic acid, methodology study

CLC Number:

TQ353.7

Qiang ZHANG,Hongfu JI,Yan ZHOU,Yu FAN,Qing GE,Zhuqian XIAO,Jianwei MAO. Monosaccharide and Alduronic Acid Composition of Bamboo Leaves by Ion Chromatography[J]. Biomass Chemical Engineering, 2019, 53(6): 33-38.

Figures/Tables 6

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References 19

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进样次数¹⁾ number of injections	岩藻糖 fucose	鼠李糖 rhammose	阿拉伯糖 arabinose	半乳糖 galactose	葡萄糖 glucose	木糖 xylose	果糖 fructose	半乳糖醛酸 galacturonic acid	葡萄糖醛酸 lucuronic acid
1	10.59	2.51	5.26	2.09	2.4	1.92	15.16	6.14	2.1
2	10.57	2.53	5.29	2.09	2.4	1.9	15.17	6.14	2.1
3	10.55	2.53	5.28	2.09	2.4	1.9	15.15	6.13	2.1
4	10.54	2.53	5.27	2.09	2.4	1.9	15.21	6.11	2.0
5	10.56	2.54	5.28	2.08	2.39	1.9	15.16	6.13	2.1
6	10.55	2.54	5.3	2.09	2.4	1.9	15.2	6.11	2.1
均值mean	10.56	2.53	5.28	2.09	2.4	1.9	15.31	6.13	2.08
RSD	0.17%	0.43%	0.27%	0.20%	0.17%	0.43%	2.09%	0.22%	1.96%

标准品 standards	线性范围/(mg·L^-1) linear range	线性方程 linear equation	R²	检测限/(mg·L^-1) limit of detection	定量限/(mg·L^-1) limit of quantification
岩藻糖fucose	0.1~9	Y=2.3264X+0.2855	0.9990	0.01	0.05
鼠李糖rhammose	0.1~9	Y=1.5988X+0.1338	0.9993	0.01	0.05
阿拉伯糖arabinose	0.1~9	Y=2.9596X+0.1629	0.9996	0.05	0.10
半乳糖galactose	0.1~9	Y=3.4926X+0.0902	0.9993	0.05	0.10
葡萄糖glucose	0.1~9	Y=4.1235X+0.1828	0.9992	0.01	0.05
木糖xylose	0.1~9	Y=3.6903X+0.2283	0.9991	0.03	1.00
果糖fructose	0.5~9	Y=1.1397X-0.0595	0.9998	1.00	3.00
半乳糖醛酸galacturonic acid	0.5~9	Y=1.1370X-0.0235	0.9993	0.10	3.00
葡萄糖醛酸glucuronic acid	0.5~9	Y=1.0499X-0.1502	0.9993	0.10	1.00

标准品 standards	峰面积peak area(n=6)		保留时间retention time(n=6)
标准品 standards	${\bar x}$±SD	RSD/%	${\bar x}$±SD	RSD/%
岩藻糖fucose	20.64±0.19	0.92	6.84±0.02	0.34
鼠李糖rhammose	14.41±0.08	0.58	12.83±0.06	0.46
阿拉伯糖arabinose	26.54±0.14	0.53	14.76±0.08	0.56
半乳糖galactose	31.34±0.12	0.39	19.81±0.13	0.68
葡萄糖glucose	36.98±0.16	0.43	22.24±0.16	0.71
木糖xylose	33.12±0.10	0.31	25.32±0.19	0.76
果糖fructose	8.33±0.07	0.84	11.25±0.09	0.80
半乳糖醛酸galacturonic acid	9.35±0.09	0.84	46.79±0.01	0.02
葡萄糖醛酸glucuronic acid	11.95±0.09	0.92	53.17±0.01	0.02

质量浓度/(mg·L^-1) mass concn.	阿拉伯糖arabinos		半乳糖galactose		葡萄糖glucose		木糖xylose
质量浓度/(mg·L^-1) mass concn.	回收率/% recovery	RSD/%	回收率/% recovery	RSD/%	回收率/% recovery	RSD/%	回收率/% recovery	RSD/%
1	98.53	0.29	103.9	0.04	118.63	0.69	104.1	1.09
2	99.9	1.16	115.93	0.24	117.47	0.36	101.77	0.65
4	100.1	0.19	110.3	0.8	115.35	0.31	100.13	0.23

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Monosaccharide and Alduronic Acid Composition of Bamboo Leaves by Ion Chromatography

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