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Biomass Chemical Engineering ›› 2019, Vol. 53 ›› Issue (2): 19-25.doi: 10.3969/j.issn.1673-5854.2019.02.004

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Transcript and Gene Expression Analysis of Chlorococcum sp. GIEC-38 with High Lipid Productivity

Jia FENG1(),Shunni ZHU1,Jin XU1,Zhongming WANG1,Zhenhong YUAN1,2,*()   

  1. 1. Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences; Key Laboratory of Renewable Energy, Chinese Academy of Sciences; Guangdong Key Laboratory of New and Renewable Energy Research and Development, Guangzhou 510640, China
    2. Collaborative Innovation Centre of Biomass Energy, Zhengzhou 450002, China
  • Received:2017-09-08 Online:2019-03-30 Published:2019-04-09
  • Contact: Zhenhong YUAN E-mail:fengjia@ms.giec.ac.cn;yuanzh@ms.giec.ac.cn
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The transcript of Chlorococcum sp. GIEC-38 which exhibited high biomass and lipid yield was sequenced. 74 605 transcripts and 65 984 genes were obtained related to 344 metabolic pathways which were active in ribosome, protein, nucleotide, RNA, carbon fixation, photosynthesis, and lipid metabolism. The lipid content of Chlorococcum sp. GIEC-38 cultured without nitrogen reached up to over 50% and compared with the original strain, 868 genes of strain cultured without nitrogen significantly up-regulated and 1 157 genes significantly down-regulated which were distributed in 41 biological functions and 71 metabolic pathways. It was found that the expressions of synthetase catalyzed intermediate products synthesis which provided for lipid acid synthesis and the key enzymes in metabolic pathways related to lipid metabolism were up-regulated significantly. All those changes promoted lipid synthesis and increased the lipid content of GIEC-38.

Key words: Chlorococcum sp., GIEC-38, metabolic pathways, gene expression, lipid

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