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bce ›› 2017, Vol. 51 ›› Issue (5): 7-14.doi: 10.3969/j.issn.1673-5854.2017.05.002

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GC-MS Analysis of Pine Needles Extracts and Toxic Effect of Their Complex Agent on Ambrostoma quadriimopressum Motschulsky

CUI Yi, WANG Haiying, HU Jiayi, FANG Jiaoyang, WANG Tingting   

  1. College of Forestry, Northeast Forestry University, Harbin 150040, China
  • Received:2016-07-20 Online:2017-09-30 Published:2017-09-30

Abstract: Fresh needle leaves extracts of Pinus sylvestris L. var. mongolica Litv.and Pinus tabuliformis Carr. var. mukdensis Uyeki were respectively obtained by Soxhlet extraction method with hexane as solvent and analyzed by gas chromatography-mass spectrometry(GC-MS). The insecticidal activities of the extracts of the two needle leaves mixed pine sawdust wood vinegar on Ambrostoma quadriimopressum imago and larvae were studied separately.The results showed that 16 components were identificated from the extracts of Pinus sylvestris L. var. mongolica Litv. and the highest GC content compound was 2,2,4,4,6,8,8-heptamethyl-nonane(23.72%),followed by phenyl-acetic acid-3-hexenyl ester(8.56%).41 components were identificated from extracts of Pinus tabuliformis Carr. var. mukdensis Uyeki, and the highest GC content compound was caryophyllene(23.29%), followed by 4-epi-cubedol(11.24%). The insecticidal effects of five times diluted mixture of extracts of the two needle leaves and wood vinegar(volume ratio 1:9) on Ambrostoma quadriimopressum imago were poor, and the insecticidal effect of five times diluted mixture of extracts of needle leaves of Pinus tabuliformis Carr. var. mukdensis Uyeki and wood vinegar(volume ratio 1:9) on Ambrostoma quadriimopressum imago was better than that of wood vinegar. In the first four days, the insecticidal effect of five times diluted wood vinegar on Ambrostoma quadriimopressum larvae was better than the five times diluted mixture of extracts of Pinus tabuliformis Carr. var. mukdensis Uyeki fresh needle leaves and wood vinegar(volume ratio 1:9),the results were opposite on the fifth day.

Key words: Pinus sylvestris L. var. mongolica Litv., Pinus tabuliformis Carr. var. mukdensis Uyeki, Soxhlet extraction, wood vinegar, Ambrostoma quadriimopressum Motschulsky

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