Abstract: Oleuropein was analysized by HPLC with ODS C18 column, using volume ratio of methanol to water of 45:55 as mobile phase under UV light of 230 nm. Oleuropein composition were extracted by hot reflux extraction, using experimental design of single factor and orthogonal test. Different kinds of macropore resins were applied to test capability of absorption and desorption of oleuropein under static and dynamic state. The results indicate that 1)the optimum extraction conditions were as follows: 80% ethanol volume fraction, temperature 70℃,extraction time 3.5 h, solid-liquid ratio 1:15(g:mL), extract 2 times; 2) The best resin was AB-8 with a 32.1 mg/g absorption capability. The optimal desorption conditions were sample mass concentration 2 g/L, 70% ethanol-water eluting, flow rate 3 mL/min, 3 BV eluent dose; 3) The purification of oleuropein reached 47.90% from the raw extract of olive leaves. Total flavonoids was 16.4% and the yield was 6.43% after AB-8 purification.

Key words: olive leaves, oleuropein, extract, macropore resin, HPLC