Abstract: Strains were selected from five fuel ethanol hydrolysate fermentation. Thermostable domestication and the fermentation optimization of better strain were studied. The results showed that in the fermentation comparison of Candida shechatae, Pachysolen tannophilus, Neurospora intermedia and Neurospora sitophila with glucose as the single carbon source, Saccharomyces cerevisiae was the best for the highest ethanol yield and the shortest log phase with 6-13 h. By domestication, a thermostable strain was screened out, which performed normal growth under 42℃. By optimizing, the optimum fermentation conditions were determined for temperature 30℃, stirring rate 150 r/min, initial pH value 5.0, inoculation amount 10%. The yeast can not be directly used for ethanol fermentation hydrolysis. But it can be used after domesticated or strain improvement.

Key words: fuel ethanol, hydrolysate, thermostable S.cerevisiae, fermentation conditions