›› 2008, Vol. 42 ›› Issue (5): 5-8.
• 研究报告 • Previous Articles Next Articles
LIU Min, OUYANG-Jia, YONG Qiang, YU Shi-yuan
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Abstract: The effects of culture condition on β-glucosidase preparation by Aspergillus niger in submerged fermentation and the apparent enzymatic properties of β-glucosidase were investigated. Wheat bran was the optimal inducer of β-glucosidase among tested carbon sources. The optimal culture condition of β-glucosidase preparation by A. niger in submerged fermentation was 40mL medium in 250mL shake flask, the inoculation was 8%, the initial pH value was 5.5, the culture temperature and rotation speed of Incubator Shaker were 30℃ and 170r/min, respectively, and the highest β-glucosidase activity of 19.12IU/mL was obtained for 10 days cultivation. The optimal temperature and pH value of β-glucosidase reaction was 65℃ and 4.8, respectively. The β-glucosidase was stable under 40℃ and in the environment pH value of 3.0-5.0.
Key words: Aspergillius niger, β-glucosidase, wheat bran
CLC Number:
TQ91
Q55
LIU Min;OUYANG-Jia;YONG Qiang;YU Shi-yuan. Preparation of β-Glucosidase by Aspergillus niger in Submerged Fermentation[J]. , 2008, 42(5): 5-8.
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