Abstract: An novel affinity adsorbent(Col-Zr) was prepared by immobilizing Zr(Ⅳ) onto collagen and its adsorption behaviors for proteins were studied by using bovine serum albumin as model. It was found that the Zr(Ⅳ) in the adsorbent can withstand the extraction of water in the pH value range of 3.0-10.0 when the immobilized amount was 100mg Zr(Ⅳ)/g collagen. The adsorption capacity of BSA on Col-Zr was significantly affected by pH value of solution. The adsorption capacity arrived 550mg/g at optimal pH value (pH=5.0) when the initial concentration of BSA was 2.0g/L. Adsorption capacity increased with the rise of temperature, implying that chemical reaction is included in the adsorption process. The adsorption isotherms can be well described by the Langmuir equation. The adsorption rate data can be well fitted by the pseudo-second-order rate model, and the adsorption capacity calculated by the model is consistent with that of actual measurement.

Key words: collagen, immobilization, Zr(Ⅳ), bovine serum albumin(BSA), adsorption