Abstract: Flavonids in different parts of Eucommia ulmoides Oliver were determined by microwave extraction, Al(NO₃)₃-NaNO₂ chromogenesis, and UV spectroscopy based on reference material of rutin. The content of flavonoids in old leaf, young leaf, twig, thick twig, annual branch, trunk, trunk bark, taproot, taproot bark, and fibrous root, was 6.05%,4.65%,0.52%,0.33%,0.285%,0.23%,0.15%,0.155%,0.165%,respectively. The experimental results showed that old leaves had the highest content of flavonoids. It was indicated that old leaves of Eucommia ulmoides Oliver were worth to be developed and utilized in the next step.

Key words: Eucommia ulmoides, total flavone, microwave extraction, content determination