紫外法和皮粉法测定塔拉单宁含量的对比研究

doi:10.3969/j.issn.1673-5854.2022.06.005

Abstract

Abstract:

Five kinds of tara tannin with different contents were determined and compared by UV spectrophotometry and hide powder method, and the significance test of tannin content determination results was carried out by SPSS software. The results showed that there was no significant difference in the determination of tannin content of high-puritytaratannin(93%) measured by UV spectrophotometry and hide powder method(P>0.05).However, the experimental results of the four kinds of tara tannins with the content of 60% have significant differences(P < 0.05), and the tannin contents measured by hide powder method were all higher than those measured by UV spectrophotometry, where the difference was about 1.5%-2.0%. And the correction constant i.e., p=1.03, in the formula for determining the taratannin content by UV method was calculated. The results of UV-Vis absorption spectrum and high performance liquid chromatography analysis showed that the maximum absorption peaks of tara tannin and gallic tannin were both at 276 nm. At the same mass concentration, the absorption peak intensity of tara tannin was higher than that of gallic tannin. The peaking time of tara tannin was mainly in 20-40 min, and the peaking time of gallic tannin was mainly in 30-45 min.

Key words: UV spectrophotometry, hide powder method, vegetable tannin, tara tannin

CLC Number:

TQ35
TQ9

Lihua TANG, Liangliang ZHANG, He ZHANG. Comparison on the Determination of Tara Tannin Content with UV Spectrophotometry and Hide Powder Method[J]. Biomass Chemical Engineering, 2022, 56(6): 31-35.

Figures/Tables 3

References 18

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样品 sample	来源 source	塔拉单宁质量分数mass fraction of tara tannin/%
样品 sample	来源 source	紫外法UV spectrophotometry	皮粉法hide powder method
T1	实验室自制made in laboratory	93.46±0.424^a	93.14±0.643^a
T2	五峰赤诚Wufeng Chicheng	61.24±0.007^a	62.84±0.156^b
T3	五峰赤诚Wufeng Chicheng	56.43±0.255^a	58.42±0.311^b
T4	张家界久瑞Zhangjiajie Jiurui	63.32±0.141^a	64.84±0.240^b
T5	四川亭江Sichuan Tingjiang	60.92±0.375^a	62.75±0.552^b

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Comparison on the Determination of Tara Tannin Content with UV Spectrophotometry and Hide Powder Method

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