过氧甲酸预处理对甘蔗渣酶解和发酵的影响

doi:10.3969/j.issn.1673-5854.2022.05.005

Abstract

Abstract:

Sugarcane Bagasse(SCB) was used as raw material for enzymatic hydrolysis after pretreatd by peroxyformic acid(PAP). Ethanol was produced by fermentation with hydrolysate, and the effect of hydrogen peroxide(HPP) concentration on the efficiency of enzymatic hydrolysis and ethanol fermentation of SCB was investigated. The results showed that the delignification percentage of pretreated bagasse(PAP-SCB-1) reached 84.30% during PAP pretreatment of SCB when the volume ratio of HPP to formic acid(FAP) was 1∶1. Using the cellulase dosage of 10 FPIU/g(based on the mass of pretreated sugarcane bagasse), the glucose yield of PAP-SCB-1 after 72 h hydrolysis was 98.21%, which was 9.84 and 12.98 times higher than that of pretreated bagasse with hydrogen peroxide(HPP-SCB, glucose yield 9.11%) and formic acid(FAP-SCB, glucose yield 7.06%), respectively. The ethanol yield was 84.06% with the enzymatic hydrolysate fermentation for 24 h, which was higher than that of HPP-SCB(76.20%) and FAP-SCB(75.15%). The chemical composition, specific surface area and crystallinity of the materials before and after pretreatment were measured. The results showed that the lignin in bagasse could be significantly removed after pretreatment with PAP, and the lignin content was reduced from 21.27% without pretreatment to less than 10%. The specific surface area and crystallinity of PAP-SCB-1 were increased up to 13.01 m²/g and 54.18%, which were 10.66(11.39) and 1.11(1.15) times higher than those of FAP-SCB(HPP-SCB).

Key words: peroxyformic acid, sugarcane bagasse, cellulosic ethanol, enzymatic hydrolysis, fermentation

CLC Number:

TQ35

Ning LI, Fanyang MENG, Haiyan YANG, Zhengjun SHI, Ping ZHAO, Jing YANG. Effect of Peroxyformic Acid Pretreatment on Enzymatic Hydrolysis and Fermentation Efficiency of Sugarcane Bagasse[J]. Biomass Chemical Engineering, 2022, 56(5): 30-36.

Figures/Tables 6

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References 22

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样品 sample	固体回收率 solid retention rate	葡聚糖 glucan	木聚糖 xylan	木质素 lignin	纤维素回收率 recovery rate of cellulose	木质素脱除率 delignification percentage
SCB	100±0.25	46.36±0.46	22.51±1.04	21.27±0.97
HPP-SCB	92.98±1.31	47.99±1.15	21.04±1.09	19.13±0.62	96.25±1.14	10.06±0.54
FAP-SCB	92.76±1.54	47.81±1.02	20.86±1.07	18.60±0.44	95.66±1.58	12.55±1.25
PAP-SCB-1	84.87±1.93	53.73±1.06	22.52±1.01	3.34±0.64	98.36±1.96	84.30±1.56
PAP-SCB-4	84.86±1.88	52.20±0.95	21.85±0.98	3.89±0.59	95.50±2.12	81.71±1.78
PAP-SCB-8	84.58±2.03	51.58±0.89	20.88±1.06	8.40±0.48	94.10±1.22	60.51±1.45

样品 sample	乙醇质量浓度/(g·L^-1) mass concentration of ethanol	乙醇得率/% ethanol yield
SCB	2.58±0.27	68.82
HPP-SCB	2.95±0.44	76.20
FAP-SCB	2.81±0.56	75.15
PAP-SCB-1	17.10±0.45	84.06
PAP-SCB-4	13.46±0.26	81.31
PAP-SCB-8	10.29±0.34	78.37

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Effect of Peroxyformic Acid Pretreatment on Enzymatic Hydrolysis and Fermentation Efficiency of Sugarcane Bagasse

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