过氧化氢-乙酸联合预处理对甘蔗渣酶解和发酵的影响

doi:10.3969/j.issn.1673-5854.2022.04.004

Abstract

Abstract:

Sugarcane bagasse(SCB) was pretreated by hydrogen peroxide-acetic acid(HPAC). The pretreated SCB was used as raw material for enzymatic hydrolysis, and then the hydrolysate was fermented for ethanol. The effects of pretreatment on enzymatic hydrolysis and fermentation of SCB were discussed. The results showed that HPAC pretreatment removed 88.85% lignin and retained 90.10% cellulose in the substrate when 20 g SCB was added with 150 mL aqueous hydrogen peroxide(i.e., 75 mL hydrogen peroxide(30%) and 75 mL water) and 150 mL acetic acid(99%), the amount of sulfuric acid was 0.5% of the HPAC solution volume, and the reaction was preformed at 70 ℃ for 2 h. The enzyme accessibility of substrate was 80.30 mg/g, which increased by 38.26% and 31.08% compared with those of hydrogen peroxide pretreatment (HP/70-SCB) and acetic acid pretreatment(AC/70-SCB) under the same conditions, respectively, and the surface coverage of lignin decreased from 0.66 to 0.22. After hydrolysis with enzyme dosage of 5 FPIU/g(substrate), the yield of glucose was 87.63%, which was 6.89 and 20.62 times than those of HP/70-SCB and AC/70-SCB, respectively. The mass concentration of ethanol produced by fermentation was 7.57 g/L, which was 7.65 and 22.94 times than those of HP/70-SCB and AC/70-SCB, respectively.

Key words: sugarcane bagasse, HPAC pretreatment, delignification, enzymatic hydrolysis, fermentation

CLC Number:

TQ35

Fanyang MENG, Ning LI, Zhengjun SHI, Haiyan YANG, Ping ZHAO, Jing YANG. Effect of Hydrogen Peroxide-acetic Acid Pretreatment on Enzymatic Hydrolysis and Fermentation of Sugarcane Bagasse[J]. Biomass Chemical Engineering, 2022, 56(4): 25-31.

Figures/Tables 6

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References 22

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样品¹⁾ sample	回收率 solid residue yield	葡聚糖 glucan	木聚糖 xylan	木质素 lignin	纤维素回收率 recovery of cellulose	木质素脱除率 delignification
SCB	100±0.11	49.02±1.01	22.78±1.33	24.38±0.62
HP/70-SCB	98.00±0.73	49.70±0.92	21.52±1.22	23.57±1.66	99.35±1.03	5.28±0.23
AC/70-SCB	93.36±1.21	48.79±1.33	22.61±0.97	20.65±1.31	92.92±2.34	17.45±1.00
HPAC/30-SCB	87.70±2.01	53.61±1.13	24.71±0.85	17.58±1.56	95.91±0.98	30.27±0.97
HPAC/50-SCB	83.93±1.85	58.39±0.97	25.24±0.77	9.76±1.75	99.97±2.22	66.42±1.56
HPAC/70-SCB	60.40±1.63	74.18±0.72	21.23±0.22	2.58±0.13	91.40±2.31	95.30±0.93
HPAC/70-SCB-0.5	70.40±2.24	62.74±0.56	25.57±1.54	4.50±0.22	90.10±1.78	88.85±1.60

样品 sample	氧碳比 ratio of oxygen to carbon	表面覆盖率/% surface coverage		C—C/C—H/% (284.6 eV)	C—O/% (286.6 eV)	C＝O/% (287.6 eV)	O—C＝O/% (289 eV)
样品 sample	氧碳比 ratio of oxygen to carbon	S_L	S_C	C—C/C—H/% (284.6 eV)	C—O/% (286.6 eV)	C＝O/% (287.6 eV)	O—C＝O/% (289 eV)
SCB	0.50	66	34	55.28	31.45	6.48	6.79
HP/70-SCB	0.67	32	68	37.13	44.82	11.87	6.17
AC/70-SCB	0.50	66	34	52.50	28.76	8.52	10.21
HPAC/70-SCB-0.5	0.72	22	78	34.86	31.05	16.36	17.73

样品 sample	疏水度/(L·g^-1) hydrophobicity	酶可及度/(mg·g^-1) enzymatic accessibility
SCB	0.173±0.004	63.34±1.22
HP/70-SCB	0.159±0.009	58.08±2.54
AC/70-SCB	0.169±0.011	61.26±3.87
HPAC/30-SCB	0.155±0.014	66.49±3.66
HPAC/50-SCB	0.085±0.016	67.39±1.98
HPAC/70-SCB	0.067±0.005	84.54±3.99
HPAC/70-SCB-0.5	0.067±0.007	80.30±4.12

样品 sample	乙醇质量浓度/(g·L^-1) ethanol mass concentration	乙醇得率/% ethanol yield
SCB	—	—
HP/70-SCB	0.99±0.11	62.55±2.01
AC/70-SCB	0.33±0.23	63.02±1.23
HPAC/30-SCB	1.29±0.20	59.26±0.99
HPAC/50-SCB	5.42±0.21	63.76±2.10
HPAC/70-SCB	6.67±0.31	57.09±2.11
HPAC/70-SCB-0.5	7.57±0.19	63.11±1.78

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Effect of Hydrogen Peroxide-acetic Acid Pretreatment on Enzymatic Hydrolysis and Fermentation of Sugarcane Bagasse

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