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Biomass Chemical Engineering ›› 2020, Vol. 54 ›› Issue (6): 39-44.doi: 10.3969/j.issn.1673-5854.2020.06.007

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Study on Ethanol Extraction and Acetone Precipitation Process of Tea Saponin

Yaozhou ZHANG(), Qingyi GU, Qiong CHEN()   

  1. Biology of Pharmaceutical Engineering College, Xinyang Agriculture and Forestry University, Xinyang 464000, China
  • Received:2020-03-06 Online:2020-11-30 Published:2020-12-01
  • Contact: Qiong CHEN E-mail:23612085@qq.com;599299467@qq.com

Abstract:

Tea saponin was extracted and separated from Camellia oleifera seed cake by ethanol extraction and acetone precipitation process. Using the purity and yield of tea saponin as the indexes, the effects of ethanol volume fraction, liquid to solid ratio, extraction temperature, extraction time, extraction times, concentration degree of the extract, acetone amount and reslurrying solvent on extraction and purification technology were evaluated by single factor experiment. The optimum conditions were as follows: 95% volume fraction of ethanol for extraction solvent, ratio of liquid to solid 9:1 (mL:g), extraction temperature 70 ℃, extraction time 4 h, extraction times 2 times, the extraction solution was concentrated to just have solid precipitation, and the volume ratio of concentrated liquid to acetone was 1:4. Under these conditions, the purity and yield of tea saponin could reach up to 85.17% and 9.82% respectively. The effects of acetone, ethyl acetate, anhydrous ethanol and 95% ethanol as reslurrying solvent on improving the purity of tea saponin were not obvious.

Key words: Camellia oleifera seed cake, tea saponin, extraction, precipitation, 95% ethanol, acetone

CLC Number: