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Biomass Chemical Engineering ›› 2019, Vol. 53 ›› Issue (4): 1-8.doi: 10.3969/j.issn.1673-5854.2019.04.001

• Research Report •     Next Articles

Synthesis, Characterization and Anticoagulant Activity of Corncob Xylan Sulfate

Hongyu FAN1(),Min WEI1,2,*(),Jian ZHAO1,Jianchun JIANG1   

  1. 1. Institute of Chemical Industry of Forest Products, CAF; National Engineering Lab. for Biomass Chemical Utilization; Key Lab. of Chemical Engineering of Forest Products, National Forestry and Grassland Administration; Key Lab. of Biomass Energy and Material, Jiangsu Province, Nanjing 210042, China
    2. Research Institute of Forestry New Technology, CAF, Beijing 100091, China
  • Received:2018-03-18 Online:2019-07-30 Published:2019-08-05
  • Contact: Min WEI E-mail:fanhongyulc@163.com;nanjingwm@163.com
  • Supported by:
    国家自然科学基金资助项目(31530010);中国林科院林产化学工业研究所研究团队建设创新工程项目(LHSXKQ14)

Abstract:

A new corncob xylan sulfate(XS) was synthesized by sulfur trioxide-pyridine(SO3·Py) method with corncob xylan extracted by alkali method as raw material and LiCl as catalyst in aprotic solvent. The influences of solvent, temperature, reaction time and material ratio on the degree of substitution(DS) of XS were investigated and the optimal conditions were determined by orthogonal test. The structures and properties of sulfated polysaccharides were investigated by GPC, FT-IR, elemental analysis and 13C NMR spectroscopy. And the in vitro anticoagulant activity of XS was studied.The results indicated that the optimal conditions for sulfation were DMF as solvent, molar ratio of SO3·Py complex and hydroxyl unit of xylose 1.5:1, 55℃ and 3 h, and the degree of substitution of XS was 1.53 and the yield of XS was 78.2%. And the weight average molecular mass of the xylan sulfate product was 36 754 and the dispersion coefficient was 1.191. Structural characterization revealed that sulfate groups were successfully introduced into xylan. A preliminary study of vitro anticoagulant activity was conducted and the results showed that the xylan sulfate had anticoagulant activity, which could significantly prolong the APTT and TT. When the degree of substitution was 1.53 and the mass concentration was 20 mg/L, the APTT, PT and TT of XS were 36.37, 14.22 and 14.70 s, respectively, which were basically equivalent to the positive control heparin sodium.

Key words: corncob, aprotic solvents, xylan, sulfate, anticoagulant

CLC Number: