桑叶乙醇提取物的体外抗氧化与抑菌活性

doi:10.3969/j.issn.1673-5854.2019.02.006

Abstract

Abstract:

Dry mulberry leaves was extracted by soxhlet extraction to obtain ethanol extract of mulberry leaves. And the composition, antioxidant activity and antibacterial activity of the ethanol extract of mulberry leaves in vitro were measured. The results showed that the ethanol extract of mulberry leaves contained many organics, including esters(82.85%), alkanes(6.31%), aromatic hydrocarbons(1.62%) and alcohols(0.21%). There was a significant linear relationship between the total reducing power and the concentration of ethanol extracts from mulberry leaves, and the extracts had good scavenging capacities of DPPH free radicals and OH free radicals with the half inhibitory concentrations(IC₅₀) of 73.07 and 104.52 mg/L, respectively. The ethanol extract of mulberry leaves had certain inhibitory effects on Bacillus subtilis, Staphylococcus aureus and Escherichia coli. Inhibitory effects were B. subtilis > S. aureus > E. coli, IC₅₀ were 4.824, 6.806 and 14.382 g/L, respectively. The antioxidant and antibacterial activity of the ethanol extract of mulberry leaves increased with the increase of sample concentration.

Key words: mulberry leaves, ethanol extract, antioxidant, antibacterial activity

CLC Number:

TQ35
TQ651

Xinyan GAO,Haiying WANG,Zhiming LIU. In Vitro Antioxidant and Antibacterial Activity of Ethanol Extract from Mulberry Leaves[J]. Biomass Chemical Engineering, 2019, 53(2): 35-40.

Figures/Tables 5

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References 18

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序号 No.	保留时间/min retention time	化合物名称 compound name	分子式 molecular formula	GC含量/% GC content	匹配度/% matching degree
1	4.945	乙酸丁酯acetic acid butyl ester	C₆H₁₂O₂	81.55	95.90
2	5.488	3-(2-甲氧基乙氧基甲氧基)-2-甲基戊烷-1-醇 3-(2-methoxyethoxymethoxy)-2-methylpentan-1-ol	C₁₀H₂₂O₄	0.21	69.10
3	5.720	乙苯ethylbenzene	C₈H₁₀	1.25	82.90
4	5.857	邻二甲苯o-xylene	C₈H₁₀	0.27	84.40
5	6.283	对二甲苯p-xylene	C₈H₁₀	0.10	66.20
6	8.126	2, 5-二甲基壬烷2, 5-dimethyl-nonane	C₁₁H₂₄	0.08	62.20
7	9.308	5-丁基壬烷5-butyl-nonane	C₁₃H₂₈	0.14	66.60
8	9.776	十一烷undecane	C₁₁H₂₄	6.09	92.50
9	20.513	邻苯二甲酸二丁酯dibutyl phthalate	C₁₆H₂₂O₄	1.30	86.00

样品 sample	质量浓度/(g·L^-1) mass concentration	自由基清除率free radical scavenging rate/%
样品 sample	质量浓度/(g·L^-1) mass concentration	DPPH·	·OH
桑叶乙醇提取物 ethanol extract from mulberryleaves	4.8	99.14±0.29	77.70±0.04
	3.6	99.00±0.10	74.92±0.23
	2.4	95.29±0.38	73.07±0.26
	1.2	94.43±0.19	68.96±0.37
	0.6	93.56±0.10	63.73±0.15
	0.3	86.88±0.29	54.61±0.56
	0.15	79.92±0.38	52.69±0.19
	0.075	61.77±0.67	49.53±0.07
抗坏血酸 ascorbic acid	4.0	100.00	59.90
	2.0	99.85	24.91
	1.0	99.60	13.37
	0.8	98.56	11.85
	0.6	97.38	10.17
	0.4	96.73	6.37
	0.2	96.07	4.13

质量浓度/(g·L^-1) mass concentration	供试菌种抑菌圈直径test strain¹⁾/mm
质量浓度/(g·L^-1) mass concentration	大肠杆菌E. coli	金黄色葡萄球菌S. aureus	枯草芽孢杆菌B. subtilis
19.2	11.96±0.92C	14.03±0.11B	15.54±0.85A
9.6	9.96±0.81B	13.35±0.64A	13.11±1.06A
4.8	9.16±0.32B	10.74±0.47B	12.64±0.51A
2.4	8.40±0.29C	9.68±0.21B	11.93±1.28A
1.2	7.70±0.46C	9.25±0.32B	10.92±0.18A
0.6	7.25±0.12B	8.76±0.31A	8.49±0.03A
0.3	6.72±0.09B	7.54±0.09A	6.00±0.00B
0.15	6.00±0.00A	6.00±0.00A	6.00±0.00A

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In Vitro Antioxidant and Antibacterial Activity of Ethanol Extract from Mulberry Leaves

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