油菜秸秆高温水解液中单糖组分的高效液相色谱测定

doi:10.3969/j.issn.1673-5854.2017.06.005

Abstract

Abstract: The method was developed to determine monosaccharides in the high temperature hydrolysate of rape straw catalyzed by CO₂ via high performance liquid chromatography(HPLC). The monosaccharides were separated on the Prevail^TM ES carbohydrate column at 35 ℃ with the mixture of acetonitrile/water(80:20,volume ratio) as mobile phase, which was under isocratic elution at the flow rate of 0.8 mL/min. They were detected by evaporative light-scattering detector(ELSD) using dry air as carrier gas at flow rate of 3.0 L/min and drift tube working at 95 ℃. The HPLC analysis method could ensure six monosaccharides(including arabinose, xylose, fructose, mannose, galactose and glucose) separate well within 20 min. The resultant correlation coefficients(R²) of monosaccharides were 0.999 1-0.999 7. The average recovery values were 95.3 %-108.0 %, and the relative standard deviation percentage(RSD) was less than 3 %.

Key words: high performance liquid chromatography, evaporative light-scattering detector, straw hydrolysate, monosaccharide

CLC Number:

TQ35

FENG Guangrong, LU Houfang, LIANG Bin, LIU Yingying. Determination of Monosaccharides in High Temperature Hydrolysate of Rape Straw by High Performance Liquid Chromatography[J]. bce, 2017, 51(6): 26-32.

References

[1] 卫新来,汪志,阮仁祥,等. 木质纤维素生物质降解产物的化学转化研究进展[J]. 现代化工,2010,30(12):26-31.
[2] SUN Y,CHENG J Y. Hydrolysis of lignocellulosic materials for ethanol production:A review[J]. Bioresource Technology,2002,83(1):1-11.
[3] ALONSO D M,BOND J Q,DUMESIC J A. Catalytic conversion of biomass to biofuels[J]. Green Chemistry,2010,12(9):1493-1513.
[4] GUPTA A,VERMA J P. Sustainable bio-ethanol production from agro-residues:A review[J]. Renewable and Sustainable Energy Reviews,2015,41:550-567.
[5] BAEYENS J,KANG Q,APPELS L,et al. Challenges and opportunities in improving the production of bio-ethanol[J]. Progress in Energy and Combustion Science,2015,47:60-88.
[6] 张名佳,苏荣欣,齐崴,等. 木质纤维素酶解糖化[J]. 化学进展,2009,21(5):1070-1074.
[7] LEE J W,JEFFRIES T W. Efficiencies of acid catalysts in the hydrolysis of lignocellulosic biomass over a range of combined severity factors[J].Bioresource Technology,2011,102(10):5884-5890.
[8] 徐明忠,庄新姝,袁振宏,等. 木质纤维素类生物质稀酸水解技术研究进展[J]. 可再生能源,2008,26(3):43-47.
[9] GAO M,XU F,LI S R,et al. Effect of SC-CO₂ pretreatment in increasing rice straw biomass conversion[J]. Biosystems Engineering,2010,106(4):470-475.
[10] NARAYANASWAMY N,FAIK A,GOETZ D J,et al. Supercritical carbon dioxide pretreatment of corn stover and switchgrass for lignocellulosic ethanol production[J]. Bioresource Technology,2011,102(13):6995-7000.
[11] ZHAO P,LU H F,LIU S J,et al. Effect of carbon dioxide on the liquid hot-water treatment of lignocellulosics[J]. Journal of Biobased Materials and Bioenergy,2015,9(3):334-341.
[12] 赵凯,许鹏举,谷广烨. 3,5-二硝基水杨酸比色法测定还原糖含量的研究[J]. 食品科学,2008,29(8):534-536.
[13] 任美玲,吕兆林,欧阳呓林,等. 气相色谱柱前衍生化测定竹叶多糖超临界CO₂提取物[J]. 食品科学,2012,33(6):215-219.
[14] PANG T,BAI C M,XU Y J,et al. Determination of sugars in tobacco leaf by HPLC with evaporative light scattering detection[J]. Journal of Liquid Chromatography & Related Technologies,2006,29(9):1281-1289.
[15] AGBLEVOR F A,HAMES B R,SCHELL D,et al. Analysis of biomass sugars using a novel HPLC method[J].Applied Biochemistry and Biotechnology,2007,136(3):309-326.
[16] 林慧,颜春荣,徐春祥,等. HPLC-ELSD法同时测定食品中的10种糖和糖醇[J]. 食品科学,2013,34(12):286-291.
[17] 程勇,李庆廷,李剑政,等. Prevail糖柱-HPLC-ELSD法测定烟草中水溶性糖[J]. 烟草科技,2010(3):32-37.
[18] FILSON P B,DAWSON-ANDOH B E. Characterization of sugars from model and enzyme-mediated pulp hydrolyzates using high-performance liquid chromatography coupled to evaporative light scattering detection[J]. Bioresource Technology,2009,100(24):6661-6664.
[19] YUAN X L,LIU S J,FENG G R,et al. Effects of ball milling on structural changes and hydrolysis of lignocellulosic biomass in liquid hot-water compressed carbon dioxide[J].Korean Journal of Chemical Engineering,2016,33(7):2134-2141.
[20] 杨俊,刘江生,蔡继宝,等. 高效液相色谱-蒸发光散射检测法测定烟草中的水溶性糖[J]. 分析化学,2005,33(11):1596-1598.
[21] 杜瑛. 毛竹的主要成分分析及催化热解研究[D]. 成都:四川大学硕士学位论文,2005.
[22] 郑春英,李庆勇,祖元刚. HPLC-ELSD法测定甘草中单糖的含量[J]. 东北林业大学学报,2006,34(2):109-110.
[23] 森德尔L R,柯克兰J J,格莱吉克J L. 实用高效液相色谱法的建立[M]. 2版. 张玉奎,王杰,张维冰,译. 北京:华文出版社,2001:28-50.
[24] 庄新姝,王树荣,袁振宏,等. 纤维素超低酸水解产物的分析[J]. 农业工程学报,2007,23(2):177-182.

Determination of Monosaccharides in High Temperature Hydrolysate of Rape Straw by High Performance Liquid Chromatography

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 6

Recommended Articles

Metrics

Comments

[1]	Qiang ZHANG,Hongfu JI,Yan ZHOU,Yu FAN,Qing GE,Zhuqian XIAO,Jianwei MAO. Monosaccharide and Alduronic Acid Composition of Bamboo Leaves by Ion Chromatography [J]. Biomass Chemical Engineering, 2019, 53(6): 33-38.
[2]	XU Man, WANG Yongmei, ZHANG Liangliang, HU Xinyu. Quantitative Determination of 3,4,5-Trimethoxybenzoic Acid [J]. bce, 2018, 52(1): 23-28.
[3]	MA Yi-cong, ZHOU Xin, MIAO Yuan-yuan, XU Yong, YONG Qiang, YU Shi-yuan. Production of Xylonic Acid from Dilute Sulfuric Acid Hydrolysates of Wheat Straw with Whole-cell Catalysis of Glucobacter oxydans [J]. bce, 2015, 49(2): 21-26.
[4]	YU Jin, YUE Yong-de, TANG Feng, YAO Xi, WANG Kun. Determination of Polysaccharides from Bamboo Leaves in Different Regions [J]. bce, 2014, 48(3): 40-43.
[5]	XU Man;CHEN Jia-hong;WANG Yong-mei;WU Dong-mei;WU Zai-song;ZHANG Liang-liang;. Quantitative Determination of Dodecyl Gallate [J]. , 2011, 45(1): 25-28.
[6]	CHEN Mu;LIAN Zhi-na;XU Yong;YONG Qiang;YU Shi-yuan;. Determination of Xylo-oligosaccharides by Sulfuric Acid Hydrolysis-High Performance Liquid Chromatography Detection [J]. , 2010, 44(6): 14-17.